Tunicamycin induced endoplasmatic reticulum changes in
endothelial cells investigated in vitro by confocal Raman imaging

Bik, E; Mielniczek, N; Jarosz, M; Denbigh, JL; Budzynska, R; Baranska, M; Majzner, K

Abstract

This paper describes how tunicamycin (Tu), the most widely used pharmacological agent for inducing endoplasmic reticulum (ER) stress, interacts with endothelial cells. Our results show that tunicamycin enters the cells and accumulates within the ER area. ER stress takes place when improperly folded or damaged proteins begin to accumulate; however, spectroscopic markers of these changes have not been identified as yet. In this work, Raman spectroscopy and scanning electron microscopy imaging of individual endothelial cells treated with Tu were performed. The changes in the biochemical composition of endothelial cells induced by Tu attributed to ER stress were studied in detail. A main feature of the Tu impact on the cells was a decrease of the phospholipid content in the area of ER, and the most abundant lipid with phosphorus groups found there, was identified as sphingomyelin.

Citation

endothelial cells investigated in vitro by confocal Raman imaging. Analyst, 144(22), 6561-6569. https://doi.org/10.1039/C9AN01456J

• Journal Article Type: Article
• Acceptance Date: Aug 27, 2019
• Online Publication Date: Aug 27, 2019
• Publication Date: Nov 21, 2019
• Deposit Date: Nov 26, 2019
• Publicly Available Date: Aug 27, 2020
• Journal: Analyst
• Print ISSN: 0003-2654
• Electronic ISSN: 1364-5528
• Publisher: Royal Society of Chemistry
• Volume: 144
• Issue: 22
• Pages: 6561-6569
• DOI: https://doi.org/10.1039/C9AN01456J
• Publisher URL: https://doi.org/10.1039/C9AN01456J
• Related Public URLs: https://www.rsc.org/journals-books-databases/about-journals/analyst/
• Additional Information: Funders : Ministry of Science and Higer Education;National Science Center; Grant Number: 0464/IP1/2016/74; Grant Number: UMO-2016/22/M/ST4/00150)