All Outputs (17)

Plasma interleukin responses as predictors of outcome stratification in patients after major trauma: a prospective observational two centre study (2023)
Journal Article
Jones, M. A., Hanison, J., Apreutesei, R., Allarakia, B., Namvar, S., Ramaswamy, D. S., …Nirmalan, N. (2023). Plasma interleukin responses as predictors of outcome stratification in patients after major trauma: a prospective observational two centre study. Frontiers in Immunology, 14, 1276171. https://doi.org/10.3389/fimmu.2023.1276171

Background and objectives: There is a need to develop objective risk stratification tools to define efficient care pathways for trauma patients. Biomarker-based point of care testing may strengthen existing clinical tools currently available for this... Read More about Plasma interleukin responses as predictors of outcome stratification in patients after major trauma: a prospective observational two centre study.

Evaluation of 4-Aminoquinoline Hydrazone Analogues as Potential Leads for Drug-Resistant Malaria (2023)
Journal Article
Magwaza, R. N., Abubaker, M., Hussain, B., Haley, M., Couper, K., Freeman, S., & Nirmalan, N. J. (in press). Evaluation of 4-Aminoquinoline Hydrazone Analogues as Potential Leads for Drug-Resistant Malaria. Molecules, 28(18), 6471. https://doi.org/10.3390/molecules28186471

The emergence of resistance to first-line antimalarial drugs calls for the development of new therapies for drug-resistant malaria. The efficacy of quinoline-based antimalarial drugs has prompted the development of novel quinolines. A panel of 4-amin... Read More about Evaluation of 4-Aminoquinoline Hydrazone Analogues as Potential Leads for Drug-Resistant Malaria.

Selected derivatives of erythromycin B- in silico and anti-malarial studies (2021)
Journal Article
Bhadra, P., Magwaza, R., Nirmalan, N., Freeman, S., Barber, J., & Arsic, B. (2021). Selected derivatives of erythromycin B- in silico and anti-malarial studies. Materials, 14(22), e6980. https://doi.org/10.3390/ma14226980

Erythromycin A is an established anti-bacterial agent against Gram-positive bacteria, but it is unstable to acid. This led to an evaluation of erythromycin B and its derivatives because these have improved acid stability. These compounds were investi... Read More about Selected derivatives of erythromycin B- in silico and anti-malarial studies.

Improved biopharmaceutical attributes of lumefantrine using choline mimicking drug delivery system : preclinical investigation on NK-65 P.berghei murine model (2021)
Journal Article
Kaur, R., Gorki, V., Katare, O., Dhingra, N., Chauhan, M., Kaur, R., …Singh, B. (2021). Improved biopharmaceutical attributes of lumefantrine using choline mimicking drug delivery system : preclinical investigation on NK-65 P.berghei murine model. Expert Opinion on Drug Delivery, 18(10), 1533-1552. https://doi.org/10.1080/17425247.2021.1946512

Background Lumefantrine (LMF) is first-line antimalarial drug, possesses activity against almost all human malarial parasites, but the in vivo activity of this molecule gets thwarted due to its low and inconsistent oral bioavailability (i.e. 4–12%)... Read More about Improved biopharmaceutical attributes of lumefantrine using choline mimicking drug delivery system : preclinical investigation on NK-65 P.berghei murine model.

Intranasal delivery of polymer-anchored lipid nanoconstructs of artemether-lumefantrine in plasmodium berghei ANKA murine model (2020)
Journal Article
Kaur, R., Gorki, V., Singh, G., Kaur, R., Katare, O., Nirmalan, N., & Singh, B. (2021). Intranasal delivery of polymer-anchored lipid nanoconstructs of artemether-lumefantrine in plasmodium berghei ANKA murine model. Journal of Drug Delivery Science and Technology, 61, 102114. https://doi.org/10.1016/j.jddst.2020.102114

Cerebral malaria is a serious plasmodial infection that calls for immediate treatment for parasitic clearance from brain. Oral regimen of cerebral malaria is an impracticable option, it being often associated with problems like impaired consciousness... Read More about Intranasal delivery of polymer-anchored lipid nanoconstructs of artemether-lumefantrine in plasmodium berghei ANKA murine model.

Homeostasis in dynamic self-regulatory physiological systems (2020)
Journal Article
Nirmalan, N., & Nirmalan, M. (2020). Homeostasis in dynamic self-regulatory physiological systems. Anaesthesia and Intensive Care Medicine, 21(11), 590-595. https://doi.org/10.1016/j.mpaic.2020.08.003

Understanding the general principles of homeostasis and its regulation in health and disease is key to managing patients in intensive care units and operating theatres. In these environments, it is crucial to realize that physiological control is a d... Read More about Homeostasis in dynamic self-regulatory physiological systems.

Hormonal control of metabolism : regulation of plasma glucose (2020)
Journal Article
Nirmalan, N., & Nirmalan, M. (2020). Hormonal control of metabolism : regulation of plasma glucose. Anaesthesia and Intensive Care Medicine, 21(11), 578-583. https://doi.org/10.1016/j.mpaic.2020.08.002

Blood glucose concentrations are required to be maintained within a narrow therapeutic range in order to ensure the normal functioning of the body. This is accomplished through a complex, interactive, finely coordinated neuro-endocrine regulatory pro... Read More about Hormonal control of metabolism : regulation of plasma glucose.

Lead optimisation of dehydroemetine for repositioned use in malaria (2020)
Journal Article
Panwar, P., Burusco, K., Abubaker, M., Matthews, H., Gutnov, A., Fernández-Álvaro, E., …Nirmalan, N. (2020). Lead optimisation of dehydroemetine for repositioned use in malaria. Antimicrobial Agents and Chemotherapy, 64(4), 1-10. https://doi.org/10.1128/AAC.01444-19

Drug repositioning offers an effective alternative to de novo drug design to tackle the urgent need for novel anti-malarial treatments. The anti-amoebic compound, emetine dihydrochloride, has been identified as a potent in-vitro inhibitor of the mult... Read More about Lead optimisation of dehydroemetine for repositioned use in malaria.

Investigating antimalarial drug interactions of emetine dihydrochloride hydrate using CalcuSyn-based interactivity calculations (2017)
Journal Article
Matthews, H., Deakin, J., Rajab, M., Idris-Usman, M., & Nirmalan, N. (2017). Investigating antimalarial drug interactions of emetine dihydrochloride hydrate using CalcuSyn-based interactivity calculations. PLoS ONE, 12(3), e0173303. https://doi.org/10.1371/journal.pone.0173303

The widespread introduction of artemisinin-based combination therapy has contributed to recent reductions in malaria mortality. Combination therapies have a range of advantages, including synergism, toxicity reduction, and delaying the onset of res... Read More about Investigating antimalarial drug interactions of emetine dihydrochloride hydrate using CalcuSyn-based interactivity calculations.

Bio-inspired artemether loaded human serum albumin nanoparticles for effective control of malaria-infected erythrocytes (2016)
Journal Article
Sidhaye, A., Bhuran, K., Zambare, S., Abubaker, M., Nirmalan, N., & Singh, K. (2016). Bio-inspired artemether loaded human serum albumin nanoparticles for effective control of malaria-infected erythrocytes. Nanomedicine, 11(21), 2809-2828. https://doi.org/10.2217/nnm-2016-0235

Background: The intra-erythrocytic development of the malarial parasite is dependent on active uptake of nutrients, including human serum albumin(HSA), into parasitized erythrocytes(pRBCs). We have designed HSA-based nanoparticles as a potential drug... Read More about Bio-inspired artemether loaded human serum albumin nanoparticles for effective control of malaria-infected erythrocytes.

“Omics”-Informed drug and biomarker discovery : opportunities, challenges and future perspectives (2016)
Journal Article
Matthews, H., Hanison, J., & Nirmalan, N. (2016). “Omics”-Informed drug and biomarker discovery : opportunities, challenges and future perspectives. Proteomes, 4(3), 28. https://doi.org/10.3390/proteomes4030028

The pharmaceutical industry faces unsustainable program failure despite significant increases in investment. Dwindling discovery pipelines, rapidly expanding R&D budgets and increasing regulatory control, predict significant gaps in the future drug m... Read More about “Omics”-Informed drug and biomarker discovery : opportunities, challenges and future perspectives.

Initial development and validation of a novel extraction method for quantitative mining of the formalin-fixed, paraffin-embedded tissue proteome for biomarker investigations (2011)
Journal Article
Nirmalan, N., Hughes, C., Peng, J., McKenna, T., Langridge, J., Cairns, D., …Banks, R. (2011). Initial development and validation of a novel extraction method for quantitative mining of the formalin-fixed, paraffin-embedded tissue proteome for biomarker investigations. Journal of Proteome Research, 10(2), 896-906. https://doi.org/10.1021/pr100812d

Annotated formalin-fixed, paraffin-embedded (FFPE) tissue archives constitute a valuable resource for retrospective biomarker discovery. However, proteomic exploration of archival tissue is impeded by extensive formalin-induced covalent cross-lin... Read More about Initial development and validation of a novel extraction method for quantitative mining of the formalin-fixed, paraffin-embedded tissue proteome for biomarker investigations.

Development and validation of a novel protein extraction methodology for quantitation of protein expression in formalin-fixed paraffin-embedded tissues using western blotting (2009)
Journal Article
Nirmalan, N., Harnden, P., Selby, P., & Banks, R. (2009). Development and validation of a novel protein extraction methodology for quantitation of protein expression in formalin-fixed paraffin-embedded tissues using western blotting. The Journal of pathology, 217(4), 497-506. https://doi.org/10.1002/path.2504

The development of efficient formaldehyde cross-link reversal strategies will make the vast diagnostic tissue archives of pathology departments amenable to prospective and retrospective translational research, particularly in biomarker-driven prote... Read More about Development and validation of a novel protein extraction methodology for quantitation of protein expression in formalin-fixed paraffin-embedded tissues using western blotting.

Mining the archival formalin-fixed paraffin-embedded tissue proteome: opportunities and challenges (2008)
Journal Article
Nirmalan, N., Harnden, P., Selby, P., & Banks, R. (2008). Mining the archival formalin-fixed paraffin-embedded tissue proteome: opportunities and challenges. Molecular BioSystems, 4(7), 712-720. https://doi.org/10.1039/b800098k

The significant potential of tissue-based proteomic biomarker studies can be restricted by difficulties in accessing samples in optimal fresh-frozen form. While archival formalinfixed tissue collections with attached clinical and outcome data repre... Read More about Mining the archival formalin-fixed paraffin-embedded tissue proteome: opportunities and challenges.

Quantitative proteomics of the human malaria parasite Plasmodium falciparum and its application to studies of development and inhibition (2004)
Journal Article
Nirmalan, N., Sims, P. F. G., & Hyde, J. E. Quantitative proteomics of the human malaria parasite Plasmodium falciparum and its application to studies of development and inhibition. Molecular Microbiology, 52(4), 1187-1199. https://doi.org/10.1111/j.1365-2958.2004.04049.x

The ability to measure accurately comparative levels of protein expression after drug challenge, metabolic stress, developmental programming or other perturbation represents one of the most important goals in post-genomics malaria research. We descri... Read More about Quantitative proteomics of the human malaria parasite Plasmodium falciparum and its application to studies of development and inhibition.

Transcriptional analysis of genes encoding enzymes of the folate pathway in the human malaria parasite Plasmodium falciparum: Folate pathway transcription in malaria parasites (2002)
Journal Article
Nirmalan, N., Wang, P., Sims, P. F. G., & Hyde, J. E. (2002). Transcriptional analysis of genes encoding enzymes of the folate pathway in the human malaria parasite Plasmodium falciparum: Folate pathway transcription in malaria parasites. Molecular Microbiology, 46(1), 179-190. https://doi.org/10.1046/j.1365-2958.2002.03148.x

Folate metabolism in Plasmodium falciparum is essential for cell growth and replication, and the target of important antimalarial agents. The pathway comprises a series of enzymes that convert GTP to derivatives of tetrahydrofolate, which are cofacto... Read More about Transcriptional analysis of genes encoding enzymes of the folate pathway in the human malaria parasite Plasmodium falciparum: Folate pathway transcription in malaria parasites.

Drug repositioning as a route to anti-malarial drug discovery: preliminary investigation of the in vitro anti-malarial efficacy of emetine dihydrochloride hydrate
Journal Article
Matthews, H., Usman-Idris, M., Khan, F., Read, M., & Nirmalan, N. Drug repositioning as a route to anti-malarial drug discovery: preliminary investigation of the in vitro anti-malarial efficacy of emetine dihydrochloride hydrate. Malaria Journal, 12(1), 359. https://doi.org/10.1186/1475-2875-12-359

Background Drug repurposing or repositioning refers to the usage of existing drugs in diseases other than those it was originally used for. For diseases like malaria, where there is an urgent need for active drug candidates, the strategy offers a... Read More about Drug repositioning as a route to anti-malarial drug discovery: preliminary investigation of the in vitro anti-malarial efficacy of emetine dihydrochloride hydrate.