In this study, we have utilized a novel strategy based upon the use of dimethyl sulfoxide (DMSO) and gas chromatography-mass spectrometry (GC-MS)for the detectionand identification ofspin-trapped free radicals. Hydroxymethyl (.CH2OH) radicals, genera... Read More about A novel approach to the analysis of spin-trapped free radicals using dimethyl sulfoxide and Gas Chromatography – Mass Spectrometry (GC-MS) with both solvent extraction and headspace solid phase microextraction (HS-SPME).
All Outputs (15)
Free radical induced oxidative stress has been reported to be involved in several diseases such as diabetes mellitus, neurodegenerative disorders, cardiovascular diseases (atherosclerosis and hypertension), respiratory diseases (asthma), cataract dev... Read More about The detection and characterisation of novel alkyl spin-adducts of PBN and its derivatives using capillary gas chromatography coupled to electron-ionisation mass spectrometry.
Detection and identification of ethanal-derived spin-trapped free radicals using headspace thermal desorption gas chromatography-mass spectrometry (TD-GC-MS) (2020)
Journal Article
Manzoor, K., Mishra, S., & Podmore, I. (2020). Detection and identification of ethanal-derived spin-trapped free radicals using headspace thermal desorption gas chromatography-mass spectrometry (TD-GC-MS). Free Radical Research, 54(10), 745-755. https://doi.org/10.1080/10715762.2020.1841183In this study, we demonstrate a novel approach to the detection and identification of the
products of spin-trapped free radicals. Hydroxyl free radicals were generated by Fenton-based
chemistry in the presence of ethanal and the spin-trapping agent... Read More about Detection and identification of ethanal-derived spin-trapped free radicals using headspace thermal desorption gas chromatography-mass spectrometry (TD-GC-MS).
Certain oxygen free radicals are produced constantly in cells by metabolism and
exogenous agents. Some, such as the hydroxyl radical, may react with various
biomolecules like DNA, lipids or proteins, and thus be involved in the pathogenesis of
var... Read More about Detection and analysis of spin trapped radical adducts using thermal desorption gas chromatography mass spectrometry (TD-GC-MS).
Solid phase microextraction (SPME) method development in analysis of volatile organic compounds (VOCs) as potential biomarkers of cancer (2015)
Journal Article
The analysis of volatile organic compounds [VOCs] is an attractive approach to the discovery of potential cancer biomarkers due to its non-invasive nature and potential low costs of sampling and analysis. Solid phase microextraction [SPME] is one of... Read More about Solid phase microextraction (SPME) method development in analysis of volatile organic compounds (VOCs) as potential biomarkers of cancer.
Current challenges in volatile organic compounds analysis as potential biomarkers of cancer (2015)
Journal Article
An early diagnosis and appropriate treatment are crucial in reducing mortality among people suffering from cancer. There is a lack of characteristic early clinical symptoms in most forms of cancer, which highlights the importance of investigating new... Read More about Current challenges in volatile organic compounds analysis as potential biomarkers of cancer.
The aim of this project was to investigate the cellular biochemical and experimental therapeutic effects of L-glutamine (gin) and L-asparagine (asn) depletion for
paediatric sarcoma cells. The work presented in this thesis describes preclinical stud... Read More about Cellular biochemical and experimental therapeutic studies of L-glutamine and L-asparagine depletion for paediatric sarcoma cells.
Biochemical changes and cytotoxicity associated with methionine depletion in paediatric central nervous system tumour cell lines (2009)
Journal Article
The aim of this study was to investigate the importance of the extent and duration of methionine depletion as a cause of cytotoxicity for CNS tumour cell lines, and also to investigate the associated in vitro cellular biochemical responses. MATERIALS... Read More about Biochemical changes and cytotoxicity associated with methionine depletion in paediatric central nervous system tumour cell lines.
Methionine restriction reduces the chemosensitivity of central nervous system tumour cell lines (2009)
Journal Article
The aim of this study was to investigate the effects of methionine depletion with cytotoxic agents that are potentially influenced by depletion of methionine, and are known to have a role in CNS tumour treatments for children. MATERIALS AND METHODS:... Read More about Methionine restriction reduces the chemosensitivity of central nervous system tumour cell lines.
A study of the cytotoxic effects of methionine depletion in human brain tumour cell lines (2007)
Thesis
The aim of this project was to investigate the importance of methionine depletion as a cause
of cytotoxicity for paediatric CNS tumour cell lines, and also to investigate the in vitro
cellular biochemical responses, as measured by changes in cellul... Read More about A study of the cytotoxic effects of methionine depletion in human brain tumour cell lines.
Validation of a novel ELISA for measurement of MDA-LDL in human plasma (2003)
Journal Article
The involvement of oxidatively modified low density lipoprotein (LDL) in the development of CHD is widely described. We have produced two antibodies, recognizing the lipid oxidation product malondialdehyde (MDA) on whole LDL or ApoB-100. The antibodi... Read More about Validation of a novel ELISA for measurement of MDA-LDL in human plasma.
Quantification of UVR-induced DNA damage: global- versus gene-specific levels of thymine dimers (2003)
Journal Article
Karakoula, A., Evans, M., Podmore, I., Hutchinson, P., Lunec, J., & Cooke, M. (2003). Quantification of UVR-induced DNA damage: global- versus gene-specific levels of thymine dimers. Journal of Immunological Methods, 277(1-2), 27-37. https://doi.org/10.1016/S0022-1759%2803%2900122-4The induction and repair of DNA damage has been shown to occur heterogeneously throughout the mammalian genome. As a consequence, analysis of these parameters at a global genome level may not reflect important gene-level events. Few techniques have b... Read More about Quantification of UVR-induced DNA damage: global- versus gene-specific levels of thymine dimers.
Novel monoclonal antibody recognition of oxidative DNA damage adduct, deoxycytidine-glyoxal (2003)
Journal Article
Mistry, N., Podmore, I., Cooke, M., Butler, P., Griffiths, H., Herbert, K., & Lunec, J. (2003). Novel monoclonal antibody recognition of oxidative DNA damage adduct, deoxycytidine-glyoxal. Laboratory Investigation, 83(2), 241-250. https://doi.org/10.1097/01.LAB0000053915.88556.EDGlyoxal, a reactive aldehyde, is a decomposition product of lipid hydroperoxides, oxidative deoxyribose breakdown, or autoxidation of sugars, such as glucose. It readily forms DNA adducts, generating potential carcinogens such as glyoxalated deoxycyt... Read More about Novel monoclonal antibody recognition of oxidative DNA damage adduct, deoxycytidine-glyoxal.
Measurement of 4-hydroxy-2,2,6,6-tetramethyl-piperidine-N-oxyl by high performance liquid chromatography-electrospray ionisation mass spectrometry (2002)
Journal Article
Reverse phase high performance liquid chromatography coupled to electrospray ionisation mass spectrometry and tandem mass spectrometry has allowed quantitative measurement of a stable nitroxide, 4-hydroxy-2,2,6,6-tetramethyl-piperidine-N-oxyl (4-hydr... Read More about Measurement of 4-hydroxy-2,2,6,6-tetramethyl-piperidine-N-oxyl by high performance liquid chromatography-electrospray ionisation mass spectrometry.
In vitro analysis of volatile organic compounds in search of potential biomarkers of lung cancer
Thesis
Lung cancer is a leading cause of death from cancer worldwide. An early diagnosis and
appropriate treatment are crucial in reducing mortality among people suffering from the
disease. Therefore, one of the main focuses of lung cancer studies is on a... Read More about In vitro analysis of volatile organic compounds in search of potential biomarkers of lung cancer.