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All Outputs (20)

Overcoming drug resistance: targeting the BCL-2 family and the long non-coding RNA HCP5 in medulloblastoma and colorectal cancer (2023)
Thesis
Iseghohimen, A. (2023). Overcoming drug resistance: targeting the BCL-2 family and the long non-coding RNA HCP5 in medulloblastoma and colorectal cancer. (Thesis). University of Salford

Colorectal cancer (CRC) is one of the most common cancers in the UK and medulloblastoma is a common cancer found in children. While there has been a progressive improvement in treatment outcomes, success has been marred by drug resistance and severe... Read More about Overcoming drug resistance: targeting the BCL-2 family and the long non-coding RNA HCP5 in medulloblastoma and colorectal cancer.

Defining the role of endonuclease VIII-like 1 and 3 in the repair of interstrand crosslinks in cancer cells (2022)
Thesis
Burberry, G. Defining the role of endonuclease VIII-like 1 and 3 in the repair of interstrand crosslinks in cancer cells. (Thesis). University of Salford

Fanconi Anaemia (FA) is an inherited autosomal-recessive disorder that can lead to abnormal development, bone-marrow failure, and an increased vulnerability to carcinogenesis. Cells derived from FA patients are unusually sensitive to DNA crosslinking... Read More about Defining the role of endonuclease VIII-like 1 and 3 in the repair of interstrand crosslinks in cancer cells.

The biochemical role of the human NEIL1 and NEIL3 DNA glycosylases on model DNA replication forks (2019)
Journal Article
Albelazi, M., Martin, P., Mohammed, S., Mutti, L., Parsons, J., & Elder, R. (2019). The biochemical role of the human NEIL1 and NEIL3 DNA glycosylases on model DNA replication forks. Genes, 10(4), 315. https://doi.org/10.3390/genes10040315

Endonuclease VIII-like (NEIL) 1 and 3 proteins eliminate oxidative DNA base damage and psoralen DNA interstrand crosslinks through initiation of base excision repair. Current evidence points to a DNA replication associated repair function of NEIL1 an... Read More about The biochemical role of the human NEIL1 and NEIL3 DNA glycosylases on model DNA replication forks.

The human DNA glycosylases NEIL1 and NEIL3 excise psoralen-induced DNA-DNA cross-links in a four-stranded DNA structure (2017)
Journal Article
Martin, P., Couve, S., Zutterling, C., Albelazi, M., Groisman, R., Matkarimov, B., …Saparbaev, M. (2017). The human DNA glycosylases NEIL1 and NEIL3 excise psoralen-induced DNA-DNA cross-links in a four-stranded DNA structure. Scientific reports, 7(17438), https://doi.org/10.1038/s41598-017-17693-4

Interstrand cross-links (ICLs) are highly cytotoxic DNA lesions that block DNA replication and transcription by preventing strand separation. Previously, we demonstrated that the bacterial and human DNA glycosylases Nei and NEIL1 excise unhooked pso... Read More about The human DNA glycosylases NEIL1 and NEIL3 excise psoralen-induced DNA-DNA cross-links in a four-stranded DNA structure.

Cytotoxicity and genotoxicity of urban particulate matter in mammalian cells (2015)
Journal Article
Dumax-Vorzet, A., Tate, M., Walmsley, R., Elder, R., & Povey, A. (2015). Cytotoxicity and genotoxicity of urban particulate matter in mammalian cells. Mutagenesis, 30(5), 621-633. https://doi.org/10.1093/mutage/gev025

Ambient air particulate matter (PM)-associated reactive oxygen species (ROS) have been linked to a variety of altered cellular outcomes. In this study, three different PM samples from diesel exhaust particles (DEPs), urban dust standard reference mat... Read More about Cytotoxicity and genotoxicity of urban particulate matter in mammalian cells.

The effect of Msh2 knockdown on toxicity induced by tert-butyl-hydroperoxide, potassium bromate, and hydrogen peroxide in base excision repair proficient and deficient cells (2013)
Journal Article
Cooley, N., Elder, R., & Povey, A. (2013). The effect of Msh2 knockdown on toxicity induced by tert-butyl-hydroperoxide, potassium bromate, and hydrogen peroxide in base excision repair proficient and deficient cells. BioMed Research International, 2013, https://doi.org/10.1155/2013/152909

The DNA mismatch repair (MMR) and base excision repair (BER) systems are important determinants of cellular toxicity following exposure to agents that cause oxidative DNA damage. To examine the interactions between these different repair systems, we... Read More about The effect of Msh2 knockdown on toxicity induced by tert-butyl-hydroperoxide, potassium bromate, and hydrogen peroxide in base excision repair proficient and deficient cells.

The effect of Msh2 knockdown on methylating agent induced toxicity in DNA glycosylase deficient cells (2010)
Journal Article
Cooley, N., Elder, R., & Povey, A. (2010). The effect of Msh2 knockdown on methylating agent induced toxicity in DNA glycosylase deficient cells. Toxicology, 268(1-2), 111-117. https://doi.org/10.1016/j.tox.2009.12.008

The DNA structure recognition protein MSH2 is an important protein in DNA mismatch repair due to its role in initiating the repair process. To examine the potential interactions between mismatch repair and base excision repair (BER) we have examined... Read More about The effect of Msh2 knockdown on methylating agent induced toxicity in DNA glycosylase deficient cells.

Cells deficient in PARP-1 show an accelerated accumulation of DNA single strand breaks, but not AP sites, over the PARP-1-proficient cells exposed to MMS. (2009)
Journal Article
Pachkowski, B., Tano, K., Afonin, V., Elder, R., Takeda, S., Watanabe, M., …Nakamura, J. (2009). Cells deficient in PARP-1 show an accelerated accumulation of DNA single strand breaks, but not AP sites, over the PARP-1-proficient cells exposed to MMS. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 671(1-2), 93-9. https://doi.org/10.1016/j.mrfmmm.2009.09.006

Poly(ADP-ribose) polymerase-1 (PARP-1) is a base excision repair (BER) protein that binds to DNA single strand breaks (SSBs) and subsequently synthesizes and transfers poly(ADP-ribose) polymers to various nuclear proteins. Numerous biochemical studie... Read More about Cells deficient in PARP-1 show an accelerated accumulation of DNA single strand breaks, but not AP sites, over the PARP-1-proficient cells exposed to MMS..

Novel vectors for homologous recombination strategies in mouse embryonic stem cells: an ES cell line expressing EGFP under control of the 5T4 promoter. (2007)
Journal Article
Perez-Campo, F., Spencer, H., Elder, R., Stern, P., & Ward, C. (2007). Novel vectors for homologous recombination strategies in mouse embryonic stem cells: an ES cell line expressing EGFP under control of the 5T4 promoter. Experimental Cell Research, 313(16), 3604-15. https://doi.org/10.1016/j.yexcr.2007.07.021

The use of gene mutation/knock-out strategies in mouse embryonic stem (ES) cells has revolutionized the study of gene function in ES cells and embryonic development. However, the construction of vectors for homologous recombination strategies require... Read More about Novel vectors for homologous recombination strategies in mouse embryonic stem cells: an ES cell line expressing EGFP under control of the 5T4 promoter..

Alkylpurine-DNA-N-glycosylase excision of 7-(hydroxymethyl)-1,N6-ethenoadenine, a glycidaldehyde-derived DNA adduct (2006)
Journal Article
Wang, P., Guliaev, A., Elder, R., & Hang, B. (2006). Alkylpurine-DNA-N-glycosylase excision of 7-(hydroxymethyl)-1,N6-ethenoadenine, a glycidaldehyde-derived DNA adduct. DNA Repair, 5(1), 23-31. https://doi.org/10.1016/j.dnarep.2005.07.013

Glycidaldehyde (GDA) is a bifunctional alkylating agent that has been shown to be mutagenic in vitro and carcinogenic in rodents. However, the molecular mechanism by which it exerts these effects is not established. GDA is capable of forming exocycli... Read More about Alkylpurine-DNA-N-glycosylase excision of 7-(hydroxymethyl)-1,N6-ethenoadenine, a glycidaldehyde-derived DNA adduct.

Potential role for 8-oxoguanine DNA glycosylase in regulating inflammation (2005)
Journal Article
Mabley, J., Pacher, P., Deb, A., Wallace, R., Elder, R., & Szabó, C. (2005). Potential role for 8-oxoguanine DNA glycosylase in regulating inflammation. FASEB Journal, 19(2), 290-2. https://doi.org/10.1096/fj.04-2278fje

OGG-1 DNA glycosylase (OGG-1) is an enzyme involved in DNA repair. It excises 7,8-dihydro-8-oxoguanine, which is formed by oxidative damage of guanine. We have investigated the role of OGG-1 in inflammation using three models of inflammation: endotox... Read More about Potential role for 8-oxoguanine DNA glycosylase in regulating inflammation.

The major human AP endonuclease (Ape1) is involved in the nucleotide incision repair pathway (2004)
Journal Article
Gros, L., Ishchenko, A., Ide, H., Elder, R., & Saparbaev, M. (2004). The major human AP endonuclease (Ape1) is involved in the nucleotide incision repair pathway. Nucleic Acids Research, 32(1), 73-81. https://doi.org/10.1093/nar/gkh165

In nucleotide incision repair (NIR), an endonuclease nicks oxidatively damaged DNA in a DNA glycosylase-independent manner, providing the correct ends for DNA synthesis coupled to the repair of the remaining 5'-dangling modified nucleotide. This mech... Read More about The major human AP endonuclease (Ape1) is involved in the nucleotide incision repair pathway.

Increased formation and persistence of 1,N(6)-ethenoadenine in DNA is not associated with higher susceptibility to carcinogenesis in alkylpurine-DNA-N-glycosylase knockout mice treated with vinyl carbamate (2003)
Journal Article
Barbin, A., Wang, R., O'Connor, P., & Elder, R. (2003). Increased formation and persistence of 1,N(6)-ethenoadenine in DNA is not associated with higher susceptibility to carcinogenesis in alkylpurine-DNA-N-glycosylase knockout mice treated with vinyl carbamate. Cancer Research, 63(22), 7699-703

Ethenobases are promutagenic DNA adducts formed by some environmental carcinogens and products of endogenous lipid peroxidation. Mutation spectra in tumors induced in mice by urethane or its metabolite vinyl carbamate (Vcar) are compatible with 1,N(6... Read More about Increased formation and persistence of 1,N(6)-ethenoadenine in DNA is not associated with higher susceptibility to carcinogenesis in alkylpurine-DNA-N-glycosylase knockout mice treated with vinyl carbamate.

DNA N-glycosylase deficient mice: a tale of redundancy (2003)
Journal Article
Parsons, J., & Elder, R. (2003). DNA N-glycosylase deficient mice: a tale of redundancy. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 531(1-2), 165-75. https://doi.org/10.1016/j.mrfmmm.2003.05.001

The generation of mouse models of base excision repair deficiency has resulted in a re-examination of the cellular defence mechanisms that exist to counteract oxidative base damage. Contrary to exhibiting various detrimental effects of the gene disru... Read More about DNA N-glycosylase deficient mice: a tale of redundancy.

Compromised incision of oxidized pyrimidines in liver mitochondria of mice deficient in NTH1 and OGG1 glycosylases (2003)
Journal Article
Karahalil, B., de Souza-Pinto, N., Parsons, J., Elder, R., & Bohr, V. (2003). Compromised incision of oxidized pyrimidines in liver mitochondria of mice deficient in NTH1 and OGG1 glycosylases. Journal of Biological Chemistry, 278(36), 33701-7. https://doi.org/10.1074/jbc.M301617200

Mitochondrial DNA is constantly exposed to high levels of endogenously produced reactive oxygen species, resulting in elevated levels of oxidative damaged DNA bases. A large spectrum of DNA base alterations can be detected after oxidative stress, and... Read More about Compromised incision of oxidized pyrimidines in liver mitochondria of mice deficient in NTH1 and OGG1 glycosylases.

Repair of dihydrouracil supported by base excision repair in mNTH1 knock-out cell extracts (2002)
Journal Article
Elder, R., & Dianov, G. (2002). Repair of dihydrouracil supported by base excision repair in mNTH1 knock-out cell extracts. Journal of Biological Chemistry, 277(52), 50487-90. https://doi.org/10.1074/jbc.M208153200

In mammalian cells, thymine glycols and other oxidized pyrimidines such as 5,6-dihydrouracil are removed from DNA by the NTH1 protein, a bifunctional DNA-N-glycosylase. However, mNTH1 knock-out mice in common with other DNA glycosylase-deficient mice... Read More about Repair of dihydrouracil supported by base excision repair in mNTH1 knock-out cell extracts.

Investigating the role of NEIL3 in colorectal cancer resistance to oxaliplatin
Thesis
Iwakun, O. Investigating the role of NEIL3 in colorectal cancer resistance to oxaliplatin. (Thesis). University of Salford

Colorectal cancer (CRC) is the third most common type of cancer and accounts for 8 % of cancer related deaths worldwide. While the likelihood of colorectal carcinogenesis increases with age, genetics and lifestyle are major predisposing factors. Trea... Read More about Investigating the role of NEIL3 in colorectal cancer resistance to oxaliplatin.

The role of the human endonuclease VIII-like 1 and 3 in the repair of DNA replication blocking lesions
Thesis
Martin, P. (in press). The role of the human endonuclease VIII-like 1 and 3 in the repair of DNA replication blocking lesions. (Thesis). University of Salford

The endonuclease VIII family of DNA glycosylases initiate repair of oxidative DNA base damage through BER and have been shown to resolve DNA inter-strand crosslinks (ICLs), arising from exogenous agents. Human NEIL1 (hNEIL1) activity on psoralen gene... Read More about The role of the human endonuclease VIII-like 1 and 3 in the repair of DNA replication blocking lesions.

Expression and characterization of recombinant human NEIL3 from Escherichia coli
Thesis
Albelazi, M. (in press). Expression and characterization of recombinant human NEIL3 from Escherichia coli. (Thesis). University of Salford

The DNA glycosylase NEIL3 is one of a family of proteins that release oxidized bases from DNA, thereby initiating base excision repair. NEIL3 gene expression is normally tightly regulated and expressed only in certain rapidly dividing cells, however,... Read More about Expression and characterization of recombinant human NEIL3 from Escherichia coli.