An essential role in molting and morphogenesis of Caenorhabditis elegans for ACN-1, a novel member of the angiotensis-converting enzyme family that lacks a metallopeptidase active site (2003)
Journal Article
Brooks, D., Appleford, P., Murray, L., & Isaac, R. (2003). An essential role in molting and morphogenesis of Caenorhabditis elegans for ACN-1, a novel member of the angiotensis-converting enzyme family that lacks a metallopeptidase active site. Journal of Biological Chemistry, 278(52), 52340-52346. https://doi.org/10.1074/jbc.M308858200

Genome sequence analyses predict many proteins that are structurally related to proteases but lack catalytic residues, thus making functional assignment difficult. We show that one of these proteins (ACN-1), a unique multi-domain angiotensin-converti... Read More about An essential role in molting and morphogenesis of Caenorhabditis elegans for ACN-1, a novel member of the angiotensis-converting enzyme family that lacks a metallopeptidase active site.

Increased formation and persistence of 1,N(6)-ethenoadenine in DNA is not associated with higher susceptibility to carcinogenesis in alkylpurine-DNA-N-glycosylase knockout mice treated with vinyl carbamate (2003)
Journal Article
Barbin, A., Wang, R., O'Connor, P., & Elder, R. (2003). Increased formation and persistence of 1,N(6)-ethenoadenine in DNA is not associated with higher susceptibility to carcinogenesis in alkylpurine-DNA-N-glycosylase knockout mice treated with vinyl carbamate. Cancer Research, 63(22), 7699-703

Ethenobases are promutagenic DNA adducts formed by some environmental carcinogens and products of endogenous lipid peroxidation. Mutation spectra in tumors induced in mice by urethane or its metabolite vinyl carbamate (Vcar) are compatible with 1,N(6... Read More about Increased formation and persistence of 1,N(6)-ethenoadenine in DNA is not associated with higher susceptibility to carcinogenesis in alkylpurine-DNA-N-glycosylase knockout mice treated with vinyl carbamate.

The Caenorhabditis elegans orthologue of mammalian puromycinsensitive aminepeptidas has roles in embryogenesis and reproduction (2003)
Journal Article
Brooks, D., Hooper, N., & Isaac, R. (2003). The Caenorhabditis elegans orthologue of mammalian puromycinsensitive aminepeptidas has roles in embryogenesis and reproduction. Journal of Biological Chemistry, 278(44), 42795-42801. https://doi.org/10.1074/jbc.M306216200

Mammals possess membrane-associated and cytosolic forms of the puromycin-sensitive aminopeptidase (PSA; EC 3.4.11.14). Increasing evidence suggests the membrane PSA is involved in neuromodulation within the central nervous system and in reproductive... Read More about The Caenorhabditis elegans orthologue of mammalian puromycinsensitive aminepeptidas has roles in embryogenesis and reproduction.

DNA N-glycosylase deficient mice: a tale of redundancy (2003)
Journal Article
Parsons, J., & Elder, R. (2003). DNA N-glycosylase deficient mice: a tale of redundancy. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 531(1-2), 165-75. https://doi.org/10.1016/j.mrfmmm.2003.05.001

The generation of mouse models of base excision repair deficiency has resulted in a re-examination of the cellular defence mechanisms that exist to counteract oxidative base damage. Contrary to exhibiting various detrimental effects of the gene disru... Read More about DNA N-glycosylase deficient mice: a tale of redundancy.

Validation of a novel ELISA for measurement of MDA-LDL in human plasma (2003)
Journal Article
Bevan, R., Durand, M., Hickenbotham, P., Kitas, G., Patel, P., Podmore, I., …Lunec, J. (2003). Validation of a novel ELISA for measurement of MDA-LDL in human plasma. Free Radical Biology and Medicine, 35(5), 517-527. https://doi.org/10.1016/S0891-5849%2803%2900359-9

The involvement of oxidatively modified low density lipoprotein (LDL) in the development of CHD is widely described. We have produced two antibodies, recognizing the lipid oxidation product malondialdehyde (MDA) on whole LDL or ApoB-100. The antibodi... Read More about Validation of a novel ELISA for measurement of MDA-LDL in human plasma.

Structural requirements for the interaction of combretastatins with tubulin: how important is the trimethoxy unit? (2003)
Journal Article
Gaukroger, K., Hadfield, J., Lawrence, N., Nolan, S., & McGown, A. (2003). Structural requirements for the interaction of combretastatins with tubulin: how important is the trimethoxy unit?. Organic and Biomolecular Chemistry, 1(17), 3033-3037. https://doi.org/10.1039/b306878a

A series of combretastatins possessing both a trimethoxy unit and other substituents on ring A has been synthesised and tested for cytotoxicity and their ability to interact with the protein tubulin. All previous studies have indicated that the trime... Read More about Structural requirements for the interaction of combretastatins with tubulin: how important is the trimethoxy unit?.

Quantification of UVR-induced DNA damage: global- versus gene-specific levels of thymine dimers (2003)
Journal Article
Karakoula, A., Evans, M., Podmore, I., Hutchinson, P., Lunec, J., & Cooke, M. (2003). Quantification of UVR-induced DNA damage: global- versus gene-specific levels of thymine dimers. Journal of Immunological Methods, 277(1-2), 27-37. https://doi.org/10.1016/S0022-1759%2803%2900122-4

The induction and repair of DNA damage has been shown to occur heterogeneously throughout the mammalian genome. As a consequence, analysis of these parameters at a global genome level may not reflect important gene-level events. Few techniques have b... Read More about Quantification of UVR-induced DNA damage: global- versus gene-specific levels of thymine dimers.

Classification, follow-up and recurrence of hepatic cystic echinococcosis using ultrasound images (2003)
Journal Article
Wang, Y., Zhang, X., Bartholomot, B., Liu, B., Luo, J., Li, T., …Craig, P. (2003). Classification, follow-up and recurrence of hepatic cystic echinococcosis using ultrasound images. https://doi.org/10.1016/S0035-9203%2803%2990121-0

Ultrasound image and morphological structure of hepatic cystic echinococcosis (CE) were analysed in 277 human cases (385 hepatic hydatid cysts). These included 65 CE cases from community surveys carried out between 1995 and 2000 in 3 countries (China... Read More about Classification, follow-up and recurrence of hepatic cystic echinococcosis using ultrasound images.

Investigation of multiphoton-induced fluorescence from solutions of 5-hydroxytryptophan (2003)
Journal Article
Bisby, R., Arvanitidis, M., Botchway, C., & Clark, I. (2003). Investigation of multiphoton-induced fluorescence from solutions of 5-hydroxytryptophan. https://doi.org/10.1039/b206848f

It is reported (Shear et al, Photochem.Photobiol. 65, 931 (1997) ) that multiphoton near infrared excitation of 5-hydroxytryptophan results in a transient product with green fluorescence. Visible fluorescence from multiphoton excitation enables detec... Read More about Investigation of multiphoton-induced fluorescence from solutions of 5-hydroxytryptophan.

Novel monoclonal antibody recognition of oxidative DNA damage adduct, deoxycytidine-glyoxal (2003)
Journal Article
Mistry, N., Podmore, I., Cooke, M., Butler, P., Griffiths, H., Herbert, K., & Lunec, J. (2003). Novel monoclonal antibody recognition of oxidative DNA damage adduct, deoxycytidine-glyoxal. Laboratory Investigation, 83(2), 241-250. https://doi.org/10.1097/01.LAB0000053915.88556.ED

Glyoxal, a reactive aldehyde, is a decomposition product of lipid hydroperoxides, oxidative deoxyribose breakdown, or autoxidation of sugars, such as glucose. It readily forms DNA adducts, generating potential carcinogens such as glyoxalated deoxycyt... Read More about Novel monoclonal antibody recognition of oxidative DNA damage adduct, deoxycytidine-glyoxal.

Compromised incision of oxidized pyrimidines in liver mitochondria of mice deficient in NTH1 and OGG1 glycosylases (2003)
Journal Article
Karahalil, B., de Souza-Pinto, N., Parsons, J., Elder, R., & Bohr, V. (2003). Compromised incision of oxidized pyrimidines in liver mitochondria of mice deficient in NTH1 and OGG1 glycosylases. Journal of Biological Chemistry, 278(36), 33701-7. https://doi.org/10.1074/jbc.M301617200

Mitochondrial DNA is constantly exposed to high levels of endogenously produced reactive oxygen species, resulting in elevated levels of oxidative damaged DNA bases. A large spectrum of DNA base alterations can be detected after oxidative stress, and... Read More about Compromised incision of oxidized pyrimidines in liver mitochondria of mice deficient in NTH1 and OGG1 glycosylases.

Monte Carlo simulations of polyion−macroion complexes. 2. Polyion length and charge density dependence (2003)
Journal Article
Akinchina, A., & Linse, P. (2003). Monte Carlo simulations of polyion−macroion complexes. 2. Polyion length and charge density dependence. Journal of Physical Chemistry B, 107(32), 8011-8021. https://doi.org/10.1021/jp022460f

The complexation between a polyion and an oppositely charged spherical macroion in the framework of the primitive model has been studied by the use of Monte Carlo simulations. The polyion length, linear charge density, and bare persistence length a... Read More about Monte Carlo simulations of polyion−macroion complexes. 2. Polyion length and charge density dependence.