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Outputs (37)

Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics. (2024)
Journal Article
Krishnamurthi, R., González-Tortuero, E., Plahe, G., Goodhead, I. B., Fothergill, J. L., James, C. E., & Allison, H. E. (in press). Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics. Journal of Visualized Experiments, 10.3791/64945. https://doi.org/10.3791/64945

Temperate phages are found integrated as prophages in the majority of bacterial genomes. Some prophages are cryptic and fixed in the bacterial chromosome, but others are active and can be triggered into a replicative form either spontaneously or by e... Read More about Understanding the Impact of Temperate Bacteriophages on Their Lysogens Through Transcriptomics..

The effect of active prophage carriage on the virulence of Pseudomonas aeruginosa (2022)
Thesis
Plahe, G. The effect of active prophage carriage on the virulence of Pseudomonas aeruginosa. (Dissertation). University of Salford

The Liverpool epidemic strain (LES) of Pseudomonas aeruginosa is a key opportunistic pathogen and major cause of death in cystic fibrosis (CF) patients. The bacterium establishes biofilm communities, contributing to immune evasion and adaptation to t... Read More about The effect of active prophage carriage on the virulence of Pseudomonas aeruginosa.

Improving phage genome annotation to understand phage biology: the case of Pseudomonas aeruginosa LES prophages (2022)
Journal Article
Gonzalez Tortuero, E., Krishnamurthi, R., Goodhead, I., Allison, H., & James, C. (2022). Improving phage genome annotation to understand phage biology: the case of Pseudomonas aeruginosa LES prophages. Access Microbiology, 4(5), https://doi.org/10.1099/acmi.ac2021.po0318

Pseudomonas aeruginosais an important opportunistic pathogen, causing nosocomial infections. The Liverpool Epidemic Strain (LES), a major cause of mortality and morbidity in cystic fibrosis patients, harbours five prophages associated with increased... Read More about Improving phage genome annotation to understand phage biology: the case of Pseudomonas aeruginosa LES prophages.

Investigating the effect of prophylactic D-mannose treatment on adhesion of Uropathogenic Escherichia coli to the human bladder epithelium (2022)
Thesis

Urinary tract infection (UTI) is the second most common bacterial infection worldwide, affecting millions of people annually, mainly women, and primarily caused by Uropathogenic Escherichia coli (UPEC). Treatment of UTIs is challenged by the continue... Read More about Investigating the effect of prophylactic D-mannose treatment on adhesion of Uropathogenic Escherichia coli to the human bladder epithelium.

Comparative analysis of gene prediction tools for viral genome annotation (2021)
Journal Article
Gonzalez-Tortuero, E., Krishnamurthi, R., Allison, H., Goodhead, I., & James, C. Comparative analysis of gene prediction tools for viral genome annotation. https://doi.org/10.1101/2021.12.11.472104. Manuscript submitted for publication

The number of newly available viral genomes and metagenomes has increased exponentially since the development of high throughput sequencing platforms and genome analysis tools. Bioinformatic annotation pipelines are largely based on open reading fram... Read More about Comparative analysis of gene prediction tools for viral genome annotation.

Characterising the bacterial microbiota of Phlebotomus and Glossina insects : vectors of parasitic diseases (2021)
Thesis

As the technology used to examine bacteria using culture-independent methods has advanced, so too has our knowledge of the bacterial microbiome of insect vectors and its effect on the pathogens they carry. Trypanosomiasis and leishmaniasis are neglec... Read More about Characterising the bacterial microbiota of Phlebotomus and Glossina insects : vectors of parasitic diseases.

Establishing Sodalis species as a laboratory model of endosymbionts (2019)
Thesis
Markham, T. Establishing Sodalis species as a laboratory model of endosymbionts. (Dissertation). University of Salford

Symbiosis is defined as the close and long relationship between two organisms.
Establishment of new symbioses, or redefining relationships underpins much of the ecological diversity found in the natural world. Microbial symbionts, being some of the... Read More about Establishing Sodalis species as a laboratory model of endosymbionts.

Ram locus is a key regulator to trigger multidrug resistance in Enterobacter aerogenes (2018)
Journal Article
Molitor, A., James, C., Fanning, S., Pages, J., & Davin-Regli, A. (2018). Ram locus is a key regulator to trigger multidrug resistance in Enterobacter aerogenes. Journal of Medical Microbiology, 67(2), 148-159. https://doi.org/10.1099/jmm.0.000667

Purpose: Several genetic regulators belonging to AraC family are involved in the emergence of MDR isolates of E. aerogenes due to alterations in membrane permeability. Compared with the genetic regulator Mar, RamA may be more relevant towards the eme... Read More about Ram locus is a key regulator to trigger multidrug resistance in Enterobacter aerogenes.