Mitigation of lead toxicity in Vigna radiata genotypes by silver nanoparticles

Chen, F; Aqeel, M; Maqsood, MF; Khalid, N; Irshad, MK; Ibrahim, M; Akhter, N; Afzaal, M; Ma, J; Hashem, M; Alamri, S; Noman, A; Lam, SS

Abstract

Heavy metal (HM) contamination of the soil through anthropogenic activities influences the living systems and drastically impacts food chain. This study examined the application of silver nanoparticles (AgNPs) in two genotypes (G1 and G2) of Mung bean (Vigna radiata) for ameliorating the Pb toxicity. Different doses of Pb (0, 25, 50 μM) were differentially tackled by AgNPs with the aim of ameliorating the plant attributes. Both genotypes displayed statistically significant quantitative and qualitative modulations for Pb tolerance. In G2, the most prominent increase in plant height (43.79%), fresh biomass (49.56%) and total chlorophyll (20%) was observed at L2 (AgNPs 10 mg/L) in comparison with the control. Overall, photosynthetic rate was increased by 26% in G2 at L6 (AgNPs 25 mg/L + Pb 25 μM). In addition, the results presented 78.5% increase in water use efficiency of G2 while G1 experienced a maximum internal CO concentration (209.8%) at L8 (Pb 50 μM). AgNPs triggered balanced uptake of minerals and improved growth of Vigna genotypes. 50 μM Pb was most hazardous and caused maximum reduction in growth of Vigna plants along with a significant suppression in photosynthetic activity, increase in MDA (199.7%) in G1 and H O (292.8%) in G2. In comparison to control, maximum superoxide dismutase (376%), peroxidase (659.8%) and catalase (9.3%) activity was observed in G2 at L11. The application of AgNPs substantially enhanced plant growth and helped them in surviving well in absence as well as presence of Pb. G2 genotype exhibited substantial tolerance capability and revealed less impairment in the studied attributes than G1 and treatment of AgNPs i.e. 25 mg/L was the best level that yielded best results in both genotypes. The results demonstrate that AgNPs mediate response(s) of plants under Pb stress and particularly contributed to HM tolerance of plants and thus showing great promise for use in phytoremediation. [Abstract copyright: Copyright © 2022 Elsevier Ltd. All rights reserved.]

Citation

Chen, F., Aqeel, M., Maqsood, M., Khalid, N., Irshad, M., Ibrahim, M., …Lam, S. (2022). Mitigation of lead toxicity in Vigna radiata genotypes by silver nanoparticles. Environmental Pollution, 308, https://doi.org/10.1016/j.envpol.2022.119606

• Journal Article Type: Article
• Acceptance Date: Jun 8, 2022
• Publication Date: Jun 15, 2022
• Deposit Date: Sep 14, 2022
• Journal: Environmental Pollution
• Print ISSN: 0269-7491
• Publisher: Elsevier
• Volume: 308
• DOI: https://doi.org/10.1016/j.envpol.2022.119606
• Publisher URL: https://doi.org/10.1016/j.envpol.2022.119606
• Additional Information: Funders : National Natural Science Foundation of China;Natural Science Foundation of Jiangsu Province; Grant Number: no.51974313, 41907405; Grant Number: BK20180641