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Elimination of Off-Target Effect by Chemical Modification of 5'-End of Small Interfering RNA

Shiohama, Y; Fujita, R; Sonokawa, M; Hisano, M; Kotake, Y; Krstic- Demonacos, M; Demonacos, C; Kashiwazaki, G; Kitayama, T; Fujii, M


Y Shiohama

R Fujita

M Sonokawa

M Hisano

Y Kotake

C Demonacos

G Kashiwazaki

T Kitayama

M Fujii


In this study, the efficiency of RNA interference of small interfering RNAs (siRNAs) bearing 5′-O-methyl-2′-deoxythymidine (X) and 5′-amino-2′, 5′-dideoxythymidine (Z) at the 5′-end of the sense strand and the antisense strand of siRNA was investigated in HeLa cells stably expressing enhanced green fluorescent protein. The results indicated that when one strand of siRNA was modified with X or Z and the other was unmodified, the X or Z modification was predominant in the process of strand selection and the unmodified strand was selected as a guide strand. When both strands are modified with X or Z, the modified antisense strand with X or Z will be selected as a guide strand with a certain probability. The resulting mature RNA-induced silencing complex exerted reduced, but still moderate silencing activity remained. These results suggest that the modification of the sense strand with X or Z eliminates the off-target effects caused by the sense strand without affecting the silencing efficiency of the siRNA.


Shiohama, Y., Fujita, R., Sonokawa, M., Hisano, M., Kotake, Y., Krstic- Demonacos, M., …Fujii, M. (2022). Elimination of Off-Target Effect by Chemical Modification of 5'-End of Small Interfering RNA. Nucleic Acid Therapeutics, 32(5), 438-447.

Journal Article Type Article
Acceptance Date Mar 7, 2022
Online Publication Date Apr 11, 2022
Publication Date Apr 11, 2022
Deposit Date Jun 9, 2022
Journal Nucleic Acid Therapeutics
Print ISSN 2159-3337
Electronic ISSN 2159-3345
Publisher Mary Ann Liebert
Volume 32
Issue 5
Pages 438-447
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