E Guerin
Biology and taxonomy of crAss-like Bacteriophages, the most abundant virus in the human gut
Guerin, E; Shkoporov, A; Stockdale, SR; Clooney, AG; Ryan, FJ; Sutton, TDS; Draper, LA; Gonzalez Tortuero, E; Paul Ross, R; Hill, C
Authors
A Shkoporov
SR Stockdale
AG Clooney
FJ Ryan
TDS Sutton
LA Draper
E Gonzalez Tortuero
R Paul Ross
C Hill
Abstract
CrAssphages represent the most abundant virus in the human gut microbiota, but the lack of available genome sequences for comparison has kept them enigmatic. Recently, sequence-based classification of distantly related crAss-like phages from multiple environments was reported, leading to a proposed familial-level taxonomic group. Here, we assembled the metagenomic sequencing reads from 702 human fecal virome/phageome samples and analyzed 99 complete circular crAss-like phage genomes and 150 contigs ≥70 kb. In silico comparative genomics and taxonomic analysis enabled a classification scheme of crAss-like phages from human fecal microbiomes into four candidate subfamilies composed of ten candidate genera. Laboratory analysis was performed on fecal samples from an individual harboring seven distinct crAss-like phages. We achieved crAss-like phage propagation in ex vivo human fecal fermentations and visualized short-tailed podoviruses by electron microscopy. Mass spectrometry of a crAss-like phage capsid protein could be linked to metagenomic sequencing data, confirming crAss-like phage structural annotations.
| Journal Article Type | Article |
|---|---|
| Acceptance Date | Sep 17, 2018 |
| Online Publication Date | Oct 25, 2018 |
| Publication Date | Nov 14, 2018 |
| Deposit Date | Feb 22, 2021 |
| Journal | Cell Host & Microbe |
| Print ISSN | 1931-3128 |
| Electronic ISSN | 1934-6069 |
| Publisher | Elsevier |
| Volume | 24 |
| Issue | 5 |
| Pages | 653-664.e6 |
| DOI | https://doi.org/10.1016/j.chom.2018.10.002 |
| Publisher URL | https://doi.org/10.1016/j.chom.2018.10.002 |
| Related Public URLs | http://www.cell.com/cell-host-microbe/home |
| Additional Information | Access Information : This article can be read for free using the link above. Funders : Science Foundation Ireland (SFI);Janssen Biotech Grant Number: SFI/12/RC/2273, SFI/15/ERCD/3189, and SFI/14/SP APC/B3032 |