Microsatellite markers for bearded capuchins (Sapajus libidinosus) : transferability and characterization

Moraes, BÁLCDE; Borges, DB; Souza-Alves, JÃP; Boubli, JP; Bezerra, B

Abstract

Natural Sapajus libidinosus populations are in continuous decline due to fragmentation, habitat loss, and the illegal pet trade. They live in Caatinga scrub forests, which already lost over 50% of their original cover. The lack of studies on S. libidinosus population genetics means that we do not know how they are being affected by this striking habitat loss and other anthropogenic disturbances. Polymorphic markers are not available for the study of S. libidinosus diversity and population genetics. Thus, here we aimed to test the transferability of 14 microsatellite markers to S. libidinosus. These microsatellites were previously isolated from Cebus capucinus (white-faced capuchin), species belonging to the same subfamily (Cebinae) as the study species. We found that six of the tested microsatellite markers (tetra-nucleotide) were cross-amplified in our target species. All loci were polymorphic. The number of alleles varied from 4 to 7, and the expected heterozygosity ranged from 0.588 to 0.869. The microsatellite markers transferred to S. libidinosus and characterised in our study will be valuable tools to evaluate the genetic variability of both wild and captive populations. They will considerably reduce the costs of microsatellite isolations, helping to prioritise currently limited research and conservation budgets in Brazil.

Citation

Moraes, B., Borges, D., Souza-Alves, J., Boubli, J., & Bezerra, B. (2021). Microsatellite markers for bearded capuchins (Sapajus libidinosus) : transferability and characterization. Anais da Academia Brasileira de Ciências (Impresso), 93(2), e20190802. https://doi.org/10.1590/0001-3765202120190802

• Journal Article Type: Article
• Acceptance Date: Nov 7, 2019
• Publication Date: Jan 18, 2021
• Deposit Date: Feb 3, 2021
• Publicly Available Date: Feb 3, 2021
• Journal: Anais da Academia Brasileira de Ciencias
• Print ISSN: 0001-3765
• Electronic ISSN: 1678-2690
• Volume: 93
• Issue: 2
• Pages: e20190802
• DOI: https://doi.org/10.1590/0001-3765202120190802
• Publisher URL: https://doi.org/10.1590/0001-3765202120190802
• Related Public URLs: http://www.scielo.br/scielo.php?script=sci_serial&pid=0001-3765&lng=en&nrm=iso
• Additional Information: Additional Information : ** From PubMed via Jisc Publications Router **Journal IDs: eissn 1678-2690 **Article IDs: pubmed: 33470383; pii: S0001-37652021000300801 **History: accepted 07-11-2019; submitted 22-07-2019; Funders : Fundação de Amparo a Ciência e Tecnologia do Estado de Pernambuco (FACEPE);Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES);Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Grant Number: IBPG-1013-2.04/14; Grant Number: Finance Codes 001 and PDSE - 88881.134891/2016-01; Grant Number: PDSE - Finance Code 88881.132672/2016-01; Grant Number: 527091; Grant Number: BCT-0025-2.05/17; Grant Number: APQ-1534-2.04/10; APQ-0143-2.04/14; Grant Number: 445071/2014-1; Grant Number: 309256/2019-4