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Validation of FASTFISH-ID : a new commercial platform for rapid fish species authentication via universal closed-tube barcoding

Naaum, AM; Cusa, MLJ; Singh, M; Bleicher, Z; Elliott, C; Goodhead, IB; Hanner, RH; Mariani, S; Rice, JE; Wangh, LJ; Sanchez, JA

Authors

AM Naaum

MLJ Cusa

M Singh

Z Bleicher

C Elliott

RH Hanner

S Mariani

JE Rice

LJ Wangh

JA Sanchez



Abstract

Seafood represents up to 20% of animal protein consumption in global food consumption and is a critical dietary and income resource for the world’s population. Currently, over 30% of marine fish stocks are harvested at unsustainable levels, and the industry faces challenges related to Illegal, Unregulated and Unreported (IUU) fishing. Accurate species identification is one critical component of successful stock management and helps combat fraud. Existing DNA-based technologies permit identification of seafood even when morphological features are removed, but are either too time-consuming, too expensive, or too specific for widespread use throughout the seafood supply chain. FASTFISH-ID is an innovative commercial platform for fish species authentication, employing closed-tube barcoding in a portable device. This method begins with asymmetric PCR amplification of the full length DNA barcode sequence and subsequently interrogates the resulting single-stranded DNA with a universal set of Positive/Negative probes labeled in two fluorescent colors. Each closed-tube reaction generates two species-specific fluorescent signatures that are then compared to a cloud-based library of previously validated fluorescent signatures. This novel approach results in rapid, automated species authentication without the need for complex, time consuming, identification by DNA sequencing, or repeated analysis with a panel of species-specific tests. Performance of the FASTFISH-ID platform was assessed in a blinded study carried out in three laboratories located in the UK and North America. The method exhibited a 98% success rate among the participating laboratories when compared to species identification via conventional DNA barcoding by sequencing. Thus, FASTFISH-ID is a promising new platform for combating seafood fraud across the global seafood supply chain.

Citation

Naaum, A., Cusa, M., Singh, M., Bleicher, Z., Elliott, C., Goodhead, I., …Sanchez, J. (2021). Validation of FASTFISH-ID : a new commercial platform for rapid fish species authentication via universal closed-tube barcoding. Food Research International, 141, 110035. https://doi.org/10.1016/j.foodres.2020.110035

Journal Article Type Article
Acceptance Date Dec 15, 2020
Online Publication Date Jan 10, 2021
Publication Date Mar 1, 2021
Deposit Date Dec 16, 2020
Publicly Available Date Jan 13, 2021
Journal Food Research International
Print ISSN 0963-9969
Publisher Elsevier
Volume 141
Pages 110035
DOI https://doi.org/10.1016/j.foodres.2020.110035
Publisher URL https://doi.org/10.1016/j.foodres.2020.110035
Related Public URLs http://www.journals.elsevier.com/food-research-international/
Additional Information Funders : European Union
Projects : SEATRACES
Grant Number: EAPA87/2016

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