Temperature sensing using fluorescent nanothermometers

Martinez Maestro, L; Vetrone, F; Naccache, R; Zamarron, A; Juarranz de la Fuente, A; Sanz-Rodriguez, F; Martin Rodriguez, E; Jaque, D; García Sole, J; Capobianco, J A

Abstract

Acquiring the temperature of a single living cell is not a trivial task. In this paper, we devise a novel nanothermometer, capable of accurately determining the temperature of solutions as well as biological systems such as HeLa cancer cells. The nanothermometer is based on the temperature-sensitive fluorescence of NaYF4:Er3+,Yb3+ nanoparticles, where the intensity ratio of the green fluorescence bands of the Er3+ dopant ions (2H11/2 → 4I15/2 and 4S3/2 → 4I15/2) changes with temperature. The nanothermometers were first used to obtain thermal profiles created when heating a colloidal solution of NaYF4:Er3+,Yb3+ nanoparticles in water using a pump−probe experiment. Following incubation of the nanoparticles with HeLa cervical cancer cells and their subsequent uptake, the fluorescent nanothermometers measured the internal temperature of the living cell from 25 °C to its thermally induced death at 45 °C.

• Journal Article Type: Article
• Acceptance Date: Apr 27, 2010
• Online Publication Date: May 4, 2010
• Publication Date: Jun 22, 2010
• Deposit Date: Dec 3, 2019
• Journal: ACS Nano
• Print ISSN: 1936-0851
• Electronic ISSN: 1936-086X
• Publisher: American Chemical Society
• Volume: 4
• Issue: 6
• Pages: 3254-3258
• DOI: https://doi.org/10.1021/nn100244a
• Publisher URL: https://doi.org/10.1021/nn100244a
• Related Public URLs: https://pubs.acs.org/journal/ancac3
• Additional Information: Funders : Universidad Autonoma de Madrid;Comunidad Autonoma de Madrid;Spanish Ministerio de Educacion y Ciencia;Banco Santander-CEAL-UAM;Natural Sciences and Engineering Research Council (NSERC) of Canada;Gouvernement du Que´bec;Ministe`re du De´veloppement e´conomique, de l’Innovation et de l’Exportation;Spanish Minsterio de Educacio´n