CdSe quantum dots for two-photon fluorescence thermal imaging.

Martinez Maestro, L; Martin Rodriguez, E; Sanz Rodriguez, F; Iglesias-de la Cruz, MC; Juarranz, A; Naccache, R; Vetrone, F; Jaque, D; Capobianco, JA; Sole, JG

Abstract

The technological development of quantum dots has ushered in a new era in fluorescence bioimaging, which was propelled with the advent of novel multiphoton fluorescence microscopes. Here, the potential use of CdSe quantum dots has been evaluated as fluorescent nanothermometers for two-photon fluorescence microscopy. In addition to the enhancement in spatial resolution inherent to any multiphoton excitation processes, two-photon (near-infrared) excitation leads to a temperature sensitivity of the emission intensity much higher than that achieved under one-photon (visible) excitation. The peak emission wavelength is also temperature sensitive, providing an additional approach for thermal imaging, which is particularly interesting for systems where nanoparticles are not homogeneously dispersed. On the basis of these superior thermal sensitivity properties of the two-photon excited fluorescence, we have demonstrated the ability of CdSe quantum dots to image a temperature gradient artificially created in a biocompatible fluid (phosphate-buffered saline) and also their ability to measure an intracellular temperature increase externally induced in a single living cell.

• Journal Article Type: Article
• Online Publication Date: Nov 9, 2010
• Publication Date: Dec 8, 2010
• Deposit Date: Dec 3, 2019
• Journal: Nano Letters
• Print ISSN: 1530-6984
• Electronic ISSN: 1530-6992
• Publisher: American Chemical Society
• Volume: 10
• Issue: 12
• Pages: 5109-5115
• DOI: https://doi.org/10.1021/nl1036098
• Publisher URL: https://doi.org/10.1021/nl1036098
• Related Public URLs: https://pubs.acs.org/journal/nalefd
• Additional Information: Funders : Universidad Autónoma de Madrid and Comunidad Autonoma de Madrid;Spanish Ministerio de Educacion y Ciencia;Natural Sciences and Engineering Research Council (NSERC) of Canada;Gouvernement du Québec, Ministère du Développement économique, de l’Innovation et de l’Exportation; Grant Number: CCG087-UAM/MAT-4434 and S2009/MAT- 1756; Grant Number: MAT 2007−64686 and MAT2010-16161