Heat in optical tweezers

Martinez Maestro, L; del Rosal, B; Haro-Gonzalez, P; Ramsay, WT; Santacruz-Gomez, K; Iglesias-de la Cruz, MC; Sanz-Rodriguez, F; Chooi, JY; Rodriguez Sevilla, P; Choudhury, D; Kar, AK; Garcia Sole, J; Paterson, L; Jaque, D

Abstract

Laser-induced thermal effects in optically trapped microspheres and single cells have been investigated by Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models. Solvent absorption has been identified as the key parameter to determine laser-induced heating, which can be reduced by establishing a continuous fluid flow of the sample. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Minimum intracellular heating, well below the cytotoxic level (43 °C), has been demonstrated to occur for optical trapping with 820 nm laser radiation, thus avoiding cell damage.

• Presentation Conference Type: Other
• Conference Name: SPIE NanoScience + Engineering, 2013
• Start Date: Aug 25, 2013
• End Date: Aug 29, 2013
• Publication Date: Sep 12, 2013
• Deposit Date: Dec 5, 2019
• Publicly Available Date: Dec 5, 2019
• Book Title: Proceedings of SPIE
• ISBN: 9780819496607
• DOI: https://doi.org/10.1117/12.2027750
• Publisher URL: https://doi.org/10.1117/12.2027750
• Related Public URLs: https://spie.org/
• Additional Information: Event Type : Conference