L Martinez Maestro
Heat in optical tweezers
Martinez Maestro, L; del Rosal, B; Haro-Gonzalez, P; Ramsay, WT; Santacruz-Gomez, K; Iglesias-de la Cruz, MC; Sanz-Rodriguez, F; Chooi, JY; Rodriguez Sevilla, P; Choudhury, D; Kar, AK; Garcia Sole, J; Paterson, L; Jaque, D
Authors
B del Rosal
P Haro-Gonzalez
WT Ramsay
K Santacruz-Gomez
MC Iglesias-de la Cruz
F Sanz-Rodriguez
JY Chooi
P Rodriguez Sevilla
D Choudhury
AK Kar
J Garcia Sole
L Paterson
D Jaque
Abstract
Laser-induced thermal effects in optically trapped microspheres and single cells have been investigated by Luminescence Thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of microns, in agreement with previous theoretical models. Solvent absorption has been identified as the key parameter to determine laser-induced heating, which can be reduced by establishing a continuous fluid flow of the sample. Our experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This has been corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. Minimum intracellular heating, well below the cytotoxic level (43 °C), has been demonstrated to occur for optical trapping with 820 nm laser radiation, thus avoiding cell damage.
Citation
Martinez Maestro, L., del Rosal, B., Haro-Gonzalez, P., Ramsay, W., Santacruz-Gomez, K., Iglesias-de la Cruz, M., …Jaque, D. (2013, August). Heat in optical tweezers. Presented at SPIE NanoScience + Engineering, 2013, San Diego Convention Center
Presentation Conference Type | Other |
---|---|
Conference Name | SPIE NanoScience + Engineering, 2013 |
Conference Location | San Diego Convention Center |
Start Date | Aug 25, 2013 |
End Date | Aug 29, 2013 |
Publication Date | Sep 12, 2013 |
Deposit Date | Dec 5, 2019 |
Publicly Available Date | Dec 5, 2019 |
Book Title | Proceedings of SPIE |
ISBN | 9780819496607 |
DOI | https://doi.org/10.1117/12.2027750 |
Publisher URL | https://doi.org/10.1117/12.2027750 |
Related Public URLs | https://spie.org/ |
Additional Information | Event Type : Conference |
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