Perivascular adipose tissue contributes to the modulation of vascular tone in vivo

Saxton, SN; Withers, SB; Nyvad, J; Mazur, A; Matchkov, V; Heagerty, AM; Aalkjær, C

Abstract

Perivascular adipose tissue (PVAT) reduces vascular tone in isolated arteries in vitro, however there are no studies of PVAT effects on vascular tone in vivo. In vitro adipocyte β3-adrenoceptors play a role in PVAT function via secretion of the vasodilator adiponectin. We have investigated the effects of PVAT on vessel diameter in vivo, and the contributions of β3-adrenoceptors and adiponectin. In anaesthetised rats, sections of the intact mesenteric bed were visualised and the diameter of arteries was recorded. Arteries were stimulated with electrical field stimulation (EFS), noradrenaline (NA), arginine-vasopressin (AVP), and acetylcholine (Ach). We report that in vivo, stimulation of PVAT with EFS, NA, and AVP evokes a local anti-constrictive effect on the artery, whilst PVAT exerts a pro-contractile effect on arteries subjected to Ach. The anti-constrictive effect of PVAT stimulated with EFS and NA was significantly reduced using β3-adrenoceptor inhibition, and activation of β3-adrenoceptors potentiated the anti-constrictive effect of vessels stimulated with EFS, NA, and AVP. The β3-adrenoceptor agonist had no effect on mesenteric arteries with PVAT removed. A blocking peptide for adiponectin receptor 1 polyclonal antibody reduced the PVAT anti-constrictive effect in arteries stimulated with EFS and NA, indicating that adiponectin may be the anti-constrictive factor released upon β3-adrenoceptor activation. These results clearly demonstrate that PVAT plays a paracrine role in regulating local vascular tone in vivo, and therefore may contribute to the modulation of blood pressure. This effect is mediated via adipocyte β3-adrenoceptors, which may trigger release of the vasodilator adiponectin. [Abstract copyright: © 2019 S. Karger AG, Basel.]

Citation

Saxton, S., Withers, S., Nyvad, J., Mazur, A., Matchkov, V., Heagerty, A., & Aalkjær, C. (2019). Perivascular adipose tissue contributes to the modulation of vascular tone in vivo. Journal of Vascular Research, 56(6), 320-332. https://doi.org/10.1159/000502689

• Journal Article Type: Article
• Acceptance Date: Aug 13, 2019
• Online Publication Date: Sep 24, 2019
• Publication Date: Dec 1, 2019
• Deposit Date: Dec 5, 2019
• Journal: Journal of vascular research
• Print ISSN: 1018-1172
• Electronic ISSN: 1423-0135
• Publisher: Karger Publishers
• Volume: 56
• Issue: 6
• Pages: 320-332
• DOI: https://doi.org/10.1159/000502689
• Publisher URL: https://doi.org/10.1159/000502689
• Related Public URLs: https://www.karger.com/Journal/Home/224160
• Additional Information: Additional Information : ** From PubMed via Jisc Publications Router **Journal IDs: eissn 1423-0135 **Article IDs: pubmed: 31550717; pii: 000502689 **History: accepted 13-08-2019; submitted 07-02-2019