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Investigation into the genetic diversity in toll-like receptors 2 and 4 in the European badger Meles meles

Whiteoak, AM; Ideozu, J; Alkathiry, HA; Tomlinson, AJ; Delahay, RJ; Cowen, S; Mullineaux, E; Gormley, E; Birtles, RJ; Lun, Z-R; Hide, G

Investigation into the genetic diversity in toll-like receptors 2 and 4 in the European badger Meles meles Thumbnail


Authors

AM Whiteoak

J Ideozu

HA Alkathiry

AJ Tomlinson

RJ Delahay

S Cowen

E Mullineaux

E Gormley

Z-R Lun



Abstract

The Toll-like receptor (TLR) genes are a conserved family of genes central to the innate immune response to pathogen infection. They encode receptor proteins, recognise pathogen associated molecular patterns (PAMPs) and trigger initial immune responses. In some host-pathogen systems, it is reported that genetic differences, such as single nucleotide polymorphisms (SNPs), associate with disease resistance or susceptibility. Little is known about TLR gene diversity in the European badger (Meles meles). We collected DNA from UK badgers, carried out PCR amplification of the badger TLR2 gene and exon 3 of TLR4 and determined DNA sequences for individual badgers for TLR2 (n=61) and TLR4 exon 3 (n=59). No polymorphism was observed in TLR4. Three TLR2 amino acid haplotype variants were found. Ninety five percent of badgers were homozygous for one common haplotype (H1), the remaining three badgers had genotypes H1/H3, H1/H2 and H2/H2. By broad comparison with other species, diversity in TLR genes in badgers seems low. This could be due to a relatively localised sampling or inherent low genetic diversity. Further studies are required to assess the generality of the low observed diversity and the relevance to the immunological status of badgers.

Citation

Whiteoak, A., Ideozu, J., Alkathiry, H., Tomlinson, A., Delahay, R., Cowen, S., …Hide, G. (2018). Investigation into the genetic diversity in toll-like receptors 2 and 4 in the European badger Meles meles. Research in Veterinary Science, 119, 228-231. https://doi.org/10.1016/j.rvsc.2018.06.020

Journal Article Type Article
Acceptance Date Jun 28, 2018
Online Publication Date Jun 30, 2018
Publication Date Aug 1, 2018
Deposit Date Jul 27, 2018
Publicly Available Date Jun 30, 2019
Journal Research in Veterinary Science
Print ISSN 0034-5288
Publisher Elsevier
Volume 119
Pages 228-231
DOI https://doi.org/10.1016/j.rvsc.2018.06.020
Publisher URL https://doi.org/10.1016/j.rvsc.2018.06.020
Related Public URLs https://www.journals.elsevier.com/research-in-veterinary-science

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Version
Authors Accepted Manuscript with Table 1 and Figure 1






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