Campylobacter jejuni transcriptome changes during loss of culturability in water

Bronowski, C; Mustafa, K; Goodhead, IB; James, CE; Nelson, C; Lucaci, A; Wigley, P; Humphrey, TJ; Williams, NJ; Winstanley, C

Abstract

Background:
Water serves as a potential reservoir for Campylobacter, the leading cause of bacterial gastroenteritis in humans. However, little is understood about the mechanisms underlying variations in survival characteristics between different strains of C. jejuni in natural environments, including water.
Results:
We identified three Campylobacter jejuni strains that exhibited variability in their ability to retain culturability after suspension in tap water at two different temperatures (4°C and 25°C). Of the three strains C. jejuni M1 exhibited the most rapid loss of culturability whilst retaining viability. Using RNAseq transcriptomics, we characterised C. jejuni M1 gene expression in response to suspension in water by analyzing bacterial suspensions recovered immediately after introduction into water (Time 0), and from two sampling time/temperature combinations where considerable loss of culturability was evident, namely (i) after 24 h at 25°C, and (ii) after 72 h at 4°C. Transcript data were compared with a culture-grown control. Some gene expression characteristics were shared amongst the three populations recovered from water, with more genes being up-regulated than down. Many of the up-regulated genes were identified in the Time 0 sample, whereas the majority of down-regulated genes occurred in the 25°C (24 h) sample.
Conclusions:
Variations in expression were found amongst genes associated with oxygen tolerance, starvation and osmotic stress. However, we also found upregulation of flagellar assembly genes, accompanied by down-regulation of genes involved in chemotaxis. Our data also suggested a switch from secretion via the sec system to via the tat system, and that the quorum sensing gene luxS may be implicated in the survival of strain M1 in water. Variations in gene expression also occurred in accessory genome regions. Our data suggest that despite the loss of culturability, C. jejuni M1 remains viable and adapts via specific changes in gene expression.

Citation

Bronowski, C., Mustafa, K., Goodhead, I., James, C., Nelson, C., Lucaci, A., …Winstanley, C. (2017). Campylobacter jejuni transcriptome changes during loss of culturability in water. PLoS ONE, 12(11), e0188936. https://doi.org/10.1371/journal.pone.0188936

• Journal Article Type: Article
• Acceptance Date: Nov 13, 2017
• Online Publication Date: Nov 30, 2017
• Publication Date: Nov 30, 2017
• Deposit Date: Nov 17, 2017
• Publicly Available Date: Dec 1, 2017
• Journal: PLOS ONE
• Electronic ISSN: 1932-6203
• Publisher: Public Library of Science
• Volume: 12
• Issue: 11
• Pages: e0188936
• DOI: https://doi.org/10.1371/journal.pone.0188936
• Publisher URL: http://dx.doi.org/10.1371/journal.pone.0188936
• Related Public URLs: http://journals.plos.org/plosone/
• Additional Information: Funders : Medical Research Council (MRC);Natural Environment Research Council (NERC);Economic and Social Research Council (ESRC);Biotechnology and Biosciences Sciences Research Council (BBSRC);Food Standards Agency (FSA); Projects : ENIGMA; Grant Number: G1100799/1