A multidentate peptide for stabilization and facile bioconjugation of gold nanoparticles

Krpetic, Z; Nativo, P; Porta, F; Brust, M

Abstract

Gold nanoparticles of two different sizes stabilized by a 15-mer peptide ligand specifically designed for this purpose have been prepared in aqueous solution and characterized by UV−vis spectroscopy and TEM. The presence of the ligand and its binding mode to the particles via its four cystein thiols is evidenced by FTIR and NMR spectroscopy. Biotinylation of the particles via binding to a freely accessible lysine residue is demonstrated.

Citation

Krpetic, Z., Nativo, P., Porta, F., & Brust, M. (2009). A multidentate peptide for stabilization and facile bioconjugation of gold nanoparticles. Bioconjugate Chemistry, 20(3), 619-624. https://doi.org/10.1021/bc8003028

• Journal Article Type: Article
• Online Publication Date: Feb 16, 2009
• Publication Date: Feb 16, 2009
• Deposit Date: Oct 11, 2016
• Journal: Bioconjugate Chemistry
• Print ISSN: 1043-1802
• Publisher: American Chemical Society
• Volume: 20
• Issue: 3
• Pages: 619-624
• DOI: https://doi.org/10.1021/bc8003028
• Publisher URL: http://dx.doi.org/10.1021/bc8003028
• Related Public URLs: http://pubs.acs.org/doi/abs/10.1021/bc8003028
• Additional Information: Funders : European Comission; Projects : European Sixth Framework Project NACARDIO; Grant Number: FP6-LIFESCIHEALTH-37672