Revealing DNA interactions with exogenous agents by surface-enhanced raman scattering

Masetti, M; Xie, H; Krpetic, Z; Recanatini, M; Alvarez-Puebla, RA; Guerrini, L

Abstract

The standard protocols for DNA analysis largely involve polymerase chain reaction (PCR). However, DNA structures bound to chemical agents cannot be PCR-amplified, and therefore any sequence changes induced by external agents may be neglected. Thus, the development of analytical tools capable of characterizing the biochemical mechanisms associated with chemically induced DNA damage is demanded for the rational design of more effective chemotherapy drugs, understanding the mode of actions of carcinogenic chemicals, and monitoring the genotypic toxicology of environments. Here we report a fast, high-throughput, low-cost method for the characterization and quantitative recognition of DNA interactions with exogenous agents based on surface-enhanced Raman scattering spectroscopy. As representative chemical agents, we selected a chemotherapeutic drug (cisplatin) which forms covalent adducts with DNA, a duplex intercalating agent (methylene blue), and a cytotoxic metal ion (HgII) which inserts into T:T mismatches. Rich structural information on the DNA complex architecture and properties is provided by the unique changes of their SERS spectra, which also offer an efficient analytical tool to quantify the extent of such binding.

Citation

Masetti, M., Xie, H., Krpetic, Z., Recanatini, M., Alvarez-Puebla, R., & Guerrini, L. (2015). Revealing DNA interactions with exogenous agents by surface-enhanced raman scattering. Abstracts of papers / American Chemical Society, 137(1), 469-476. https://doi.org/10.1021/ja511398w

• Journal Article Type: Article
• Online Publication Date: Dec 15, 2014
• Publication Date: Jan 14, 2015
• Deposit Date: Oct 6, 2016
• Journal: Journal of the American Chemical Society
• Print ISSN: 0002-7863
• Volume: 137
• Issue: 1
• Pages: 469-476
• DOI: https://doi.org/10.1021/ja511398w
• Publisher URL: http://dx.doi.org/10.1021/ja511398w
• Related Public URLs: http://pubs.acs.org/doi/abs/10.1021/ja511398w
• Additional Information: Funders : European Research Council;Spanish Ministerio de Economia y Competitividad; Projects : CrossSERS;PrioSERS; Grant Number: FP7/2013 329131; Grant Number: FP7/2014 623527; Grant Number: CTQ2011-23167