Ca analysis : an Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis

Greensmith, DJ

Abstract

Here I present an Excel based program for the analysis of intracellular Ca transients recorded using fluorescent indicators. The program can perform all the necessary steps which convert recorded raw voltage changes into meaningful physiological information. The program performs two fundamental processes. (1) It can prepare the raw signal by several methods. (2) It can then be used to analyze the prepared data to provide information such as absolute intracellular Ca levels. Also, the rates of change of Ca can be measured using multiple, simultaneous regression analysis. I demonstrate that this program performs equally well as commercially available software, but has numerous advantages, namely creating a simplified, self-contained analysis workflow.

Citation

Greensmith, D. (2014). Ca analysis : an Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis. Computer Methods and Programs in Biomedicine, 113(1), 241-250. https://doi.org/10.1016/j.cmpb.2013.09.004

• Journal Article Type: Article
• Acceptance Date: Sep 9, 2013
• Online Publication Date: Sep 21, 2013
• Publication Date: Jan 1, 2014
• Deposit Date: Jan 21, 2015
• Publicly Available Date: Oct 8, 2018
• Journal: Computer methods and programs in biomedicine
• Print ISSN: 0169-2607
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 113
• Issue: 1
• Pages: 241-250
• DOI: https://doi.org/10.1016/j.cmpb.2013.09.004
• Publisher URL: http://dx.doi.org/10.1016/j.cmpb.2013.09.004
• Related Public URLs: http://www.journals.elsevier.com/computer-methods-and-programs-in-biomedicine/