Use of competition dialysis in the discovery of G-quadruplex selective ligands

Ragazzon, PA; Chaires, J

Abstract

G-quadrplex DNA can exist in a rich variety of structural forms, ranging from unimolecular folded structures containing diverse types of loops and strand oreintations, to bimolecular dimeric structures, and finally to tetramolecular parallel-stranded structures. These diverse structures present numerous potential small molecule binding sites with distinctive properties. There is mounting evidence for important functional roles for G-quadruplex structures in biology. G-quadruplexes may participate in the maintenance of telomeres, in transcriptional regulation and, in mRNA, may act to modulate translation. G-quadruplexes thus represent an attractive target for new small-molecule therapeutic agents. Competition dialysis provides a useful tool for the discovery of small molecules that selectively recognize the unique structural features of G-quadruplexes. The principles and practice of the competition dialysis experiment are described here.

Citation

Ragazzon, P., & Chaires, J. (2007). Use of competition dialysis in the discovery of G-quadruplex selective ligands. Methods, 43(4), 313-323

• Journal Article Type: Article
• Publication Date: Dec 1, 2007
• Deposit Date: Jan 26, 2015
• Journal: Methods
• Print ISSN: 1046-2023
• Electronic ISSN: 1095-9130
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 43
• Issue: 4
• Pages: 313-323
• Publisher URL: http://www.journals.elsevier.com/methods/
• Related Public URLs: http://www.ncbi.nlm.nih.gov/pubmed/17967701
• Additional Information: Funders : Brown Cancer Center