Mycobacterium microti: More diverse than previously thought

Smith, NH; Crawshaw, T; Parry, J; Birtles, RJ

Abstract

Mycobacterium microti is a member of the Mycobacterium tuberculosis complex of bacteria. This species was
originally identified as a pathogen of small rodents and shrews and was associated with limited diversity and
a much reduced spoligotype pattern. More recently, specific deletions of chromosomal DNA have been shown
to define this group of organisms, which can be identified by the absence of chromosomal region RD1mic. We
describe here the molecular characteristics of 141 strains of the Mycobacterium tuberculosis complex isolated in
Great Britain over a 14-year period. All strains have characteristic loss of some spoligotype spacers and
characteristic alleles at the ETR-E and ETR-F variable-number tandem-repeat (VNTR) loci, and a sample of
these strains was deleted for regions RD7, RD9, and RD1micbut intact for regions RD4 and RD12. We therefore
identified these strains as M. microti and show that they have much more diverse spoligotype patterns and
VNTR types than previously thought. The most common source of these strains was domestic cats, and we show
that the molecular types of M. microti are geographically localized in the same way that molecular types of
Mycobacterium bovis are geographically localized in cattle in the United Kingdom. We describe the pathology
of M. microti infection in cats and suggest that the feline disease is a spillover from a disease maintained in an
unknown wild mammal, probably field voles. The location of the cats with M. microti infection suggests that
they do not overlap geographically with the strains of Mycobacterium bovis in Great Britain

Citation

Smith, N., Crawshaw, T., Parry, J., & Birtles, R. (2009). Mycobacterium microti: More diverse than previously thought. Journal of Clinical Microbiology, 47(8), 2551-2559. https://doi.org/10.1128/JCM.00638-09

• Journal Article Type: Article
• Publication Date: Jan 1, 2009
• Deposit Date: Nov 6, 2014
• Publicly Available Date: Apr 5, 2016
• Journal: Journal of Clinical Microbiology
• Print ISSN: 0095-1137
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• Volume: 47
• Issue: 8
• Pages: 2551-2559
• DOI: https://doi.org/10.1128/JCM.00638-09
• Publisher URL: http://dx.doi.org/10.1128/JCM.00638-09
• Related Public URLs: http://jcm.asm.org/
• Additional Information: Access Information : Non-commercial; Funders : Funder not known