MafB restricts M-CSF-Dependent myeloid commitment divisions of hematopoietic stem cells

Sarrazin, S; Mossadegh-Keller, N; Fukao, T; Aziz, A; Mourcin, F; Vanhille, L; Kelly Modis, L; Kastner, P; Chan, S; Duprez, E; Otto, C; Sieweke, MH

Abstract

While hematopoietic stem cell (HSC) self-renewal is well studied, it remains unknown whether distinct control mechanisms enable HSC divisions that generate progeny cells with specific lineage bias. Here, we report that the monocytic transcription factor MafB specifically restricts the ability of M-CSF to instruct myeloid commitment divisions in HSCs. MafB deficiency specifically enhanced sensitivity to M-CSF and caused activation of the myeloid master-regulator PU.1 in HSCs in vivo. Single-cell analysis revealed that reduced MafB levels enabled M-CSF to instruct divisions producing asymmetric daughter pairs with one PU.1+ cell. As a consequence, MafB−/− HSCs showed a PU.1 and M-CSF receptor-dependent competitive repopulation advantage specifically in the myelomonocytic, but not T lymphoid or erythroid, compartment. Lineage-biased repopulation advantage was progressive, maintained long term, and serially transplantable. Together, this indicates that an integrated transcription factor/cytokine circuit can control the rate of specific HSC commitment divisions without compromising other lineages or self-renewal.

Citation

Sarrazin, S., Mossadegh-Keller, N., Fukao, T., Aziz, A., Mourcin, F., Vanhille, L., …Sieweke, M. (2009). MafB restricts M-CSF-Dependent myeloid commitment divisions of hematopoietic stem cells. Cell, 138(2), 300-313. https://doi.org/10.1016/j.cell.2009.04.057

• Journal Article Type: Article
• Publication Date: Jan 1, 2009
• Deposit Date: Sep 12, 2014
• Journal: Cell
• Print ISSN: 0092-8674
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 138
• Issue: 2
• Pages: 300-313
• DOI: https://doi.org/10.1016/j.cell.2009.04.057
• Publisher URL: http://dx.doi.org/10.1016/j.cell.2009.04.057
• Related Public URLs: http://www.cell.com/
• Additional Information: Funders : Funder not known