Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation

Elkord, E

Abstract

Cryopreservation of peripheral blood mononuclear cells (PBMC) is essential for many clinical and research assays. Some studies reported consistent changes in PBMC phenotype following cryopreservation. We hypothesized that PBMC freezing may have a negative impact on estimation of the frequency of T regulatory cell (Treg). Treg levels were measured in 6 fresh PBMC samples isolated from 6 healthy donors and these levels were re-measured after freezing for three weeks. Herein, we report a significant reduction in Treg frequency in all samples following cryopreservation.

Citation

Elkord, E. (2009). Frequency of human T regulatory cells in peripheral blood is significantly reduced by cryopreservation. Journal of Immunological Methods, 347(1-2), 87-90. https://doi.org/10.1016/j.jim.2009.06.001

• Journal Article Type: Article
• Publication Date: Aug 1, 2009
• Deposit Date: Oct 12, 2011
• Journal: Journal of Immunological Methods
• Print ISSN: 0022-1759
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 347
• Issue: 1-2
• Pages: 87-90
• DOI: https://doi.org/10.1016/j.jim.2009.06.001
• Publisher URL: http://dx.doi.org/ 10.1016/j.jim.2009.06.001