Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells

Elkord, E; Burt, D

Abstract

We here describe the development of a novel ELISPOT assay for the detection and enumeration of IFNγ-secreting functional chimeric antigen receptor (CAR)-redirected T cells against carcinoembryonic antigen (CEA). This method is valuable for clinical trials to monitor the presence of functional CEA-specific T cells transduced with a CAR. The same principle should be applicable for the detection of functional CAR-redirected T cells against any other tumour-associated antigens by immobilizing a particular biotinylated antigen to streptavidin-coated beads.

Citation

Elkord, E., & Burt, D. (2011). Novel IFNγ ELISPOT assay for detection of functional carcinoembryonic antigen-specific chimeric antigen receptor-redirected T cells. Scandinavian Journal of Immunology, 74(4), 419-422. https://doi.org/10.1111/j.1365-3083.2011.02588.x

• Journal Article Type: Article
• Publication Date: Jan 1, 2011
• Deposit Date: Oct 11, 2011
• Journal: Scandinavian Journal Of Immunology
• Print ISSN: 0300-9475
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 74
• Issue: 4
• Pages: 419-422
• DOI: https://doi.org/10.1111/j.1365-3083.2011.02588.x
• Publisher URL: http://dx.doi.org/10.1111/j.1365-3083.2011.02588.x