Tissue culture and frost tolerance studies in Solanum

Anjum, MA

Abstract

In vitro shoot cultures of two commercial cultivars of Solanum tuberosum, Desiree
and Mans Piper, and of two wild species S. commersonil and S. acaule, were
established from single nodal explants and seedling tissues respectively. Callus
cultures were initiated from potato stem, leaf and tuber explants. Cell suspension
cultures were obtained from tuber and stem-derived calluses of S. tuberosum.
Protoplasts were isolated from leaves of greenhouse-grown plants and from
suspension-cultured cells of S. tuberosum, and in vitro shoot cultures of S. tube rosum
and S. commersonii. Plantlets were regenerated from tuber discs, internodal explants,
growing calluses, suspension-cultured cells and protoplast-derived calluses.
Microtubers were induced from nodal explants of these Solanum species.
Attempts were made to select frost-tolerant cell lines through resistance to
hydroxyproline, by direct transfer of axillary buds, callus cultures and suspensioncultured
cells to media containing different concentrations of hydroxyproline.
Selection was also made after exposure of suspension-cultured cells to a freezing
temperature (-6° C), and to gamma-irradiation (20 Gy). Several hyp-resistant cell
lines were established from callus cultures and suspension-cultured cells but not from
axillary buds. Most of these selected cell lines were found to show increased
tolerance to frost. Plants were regenerated from one of the hyp-tolerant, frosttolerant
cell lines. The cellular damage to S. tuberosum cv. Desiree callus cells due
to freezing temperatures was examined, and the cellular structure of the callus of
three Solanum species and one frost-tolerant cell line was compared by electron
microscopy.

Citation

Anjum, M. Tissue culture and frost tolerance studies in Solanum. (Thesis). University of Salford

• Thesis Type: Thesis
• Deposit Date: Aug 5, 2011
• Publicly Available Date: Aug 5, 2011
• Award Date: Jan 1, 1994