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Near infrared multiphoton-induced generation and detection of hydroxyl radicals in a biochemical system

Botchway, SW; Crisostomo, AG; Parker, AW; Bisby, RH

Authors

SW Botchway

AG Crisostomo

AW Parker

RH Bisby



Abstract

Solutions of tryptophan and N-hydroxypyridine-2-thione (mercaptopyridine-N-oxide, MPNO) were irradiated at 335 nm. Formation of 5-hydroxytryptophan was inferred from increased fluorescence at 334 nm on excitation at 315 nm, conditions chosen for selective detection of 5 hydroxytryptophan. Such experiments are complicated by overlapping absorption spectra in the region of 300-350 nm. Similar solutions were exposed to multiphoton excitation at 750 nm using 180 fs pulses from a titanium:sapphire laser. In solutions containing both tryptophan and MPNO strong emission at 500 nm was observed that was absent in solutions containing either MPNO or tryptophan only. This emission is ascribed to the characteristic fluorescence (‘hyperluminescence’) from 5-hydroxyindoles resulting from multiphoton photochemistry. The conclusion that MPNO generates hydroxyl radicals by 2 photon activation at 750 nm is confirmed by the scavenging effects of ethanol and kinetic analysis of the results. This method has potential applications in intracellular induction of oxidative stress using multiphoton near-infrared illumination, a technology that is gaining momentum as a research tool.

Citation

Botchway, S., Crisostomo, A., Parker, A., & Bisby, R. (2007). Near infrared multiphoton-induced generation and detection of hydroxyl radicals in a biochemical system. Archives of Biochemistry and Biophysics, 464(2), 314-321. https://doi.org/10.1016/j.abb.2007.04.026

Journal Article Type Article
Publication Date Jan 1, 2007
Deposit Date Jun 24, 2009
Publicly Available Date Jun 24, 2009
Journal Archives of Biochemistry and Biophysics
Print ISSN 0003-9861
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 464
Issue 2
Pages 314-321
DOI https://doi.org/10.1016/j.abb.2007.04.026
Keywords Radical, hydroxyl, scavenging, multiphoton, microscopy, mercaptopyridine-n-oxide, photofenton, spectroscopy, laser, femtosecond
Publisher URL http://dx.doi.org/10.1016/j.abb.2007.04.026
Related Public URLs http://www.sciencedirect.com/science/journal/00039861
http://www.elsevier.com

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