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Nanoscale hydroxyl radical generation from multiphoton ionization of tryptophan

Bisby, RH; Crisostomo, AG; Botchway, SW; Parker, AW

Authors

RH Bisby

AG Crisostomo

SW Botchway

AW Parker



Abstract

Exposure of solutions containing both tryptophan and hydrogen peroxide to a pulsed (~180 fs) laser beam at 750 nm induces luminescence characteristic of 5 hydroxytryptophan. The results indicate that 3-photon excitation of tryptophan results in photoionization within the focal volume of the laser beam. The resulting hydrated electron is scavenged by the hydrogen peroxide to produce the hydroxyl radical. The latter subsequently reacts with tryptophan to form 5-hydroxytryptophan. The involvement of hydroxyl radicals is confirmed by use of ethanol and nitrous oxide as scavengers and their effects on the fluorescence yield in this system. It is postulated that such multiphoton ionization of tryptophanyl residues in cellular proteins may contribute to the photodamage observed during imaging of cells and tissues using multiphoton microscopy.

Citation

Bisby, R., Crisostomo, A., Botchway, S., & Parker, A. Nanoscale hydroxyl radical generation from multiphoton ionization of tryptophan. Photochemistry and Photobiology, 85(1), 353-357. https://doi.org/10.1111/j.1751-1097.2008.00447.x

Journal Article Type Article
Deposit Date Feb 16, 2009
Publicly Available Date Feb 16, 2009
Journal Photochemistry and Photobiology
Print ISSN 0031-8655
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 85
Issue 1
Pages 353-357
DOI https://doi.org/10.1111/j.1751-1097.2008.00447.x
Publisher URL http://dx.doi.org/10.1111/j.1751-1097.2008.00447.x
Related Public URLs http://eu.wiley.com/WileyCDA/Section/index.html

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