Measurement of nanosecond time-resolved fluorescence with a directly gated interline CCD camera

Mitchell, A; Wall, JE; Murray, JG; Morgan, CG

Abstract

CCD cameras coupled optically to gated image intensifiers have been used for fast time-resolved measurements for some years. Image intensifiers have disadvantages, however, and for some applications it would be better if the image sensor could be gated directly at high speed. Control of the 'charge drain' function on an interline-transfer CCD allows the sensor to be switched rapidly from an insensitive state. The temporal and spatial properties of the charge drain are explored in the present paper and it is shown that nanosecond time resolution with acceptable spatial uniformity can be achieved for a small commercial sensor. A fluorescence lifetime imaging system is demonstrated, based on a repetitively pulsed laser excitation source synchronized to the CCD control circuitry via a programmable delay unit.

Citation

Mitchell, A., Wall, J., Murray, J., & Morgan, C. (2002). Measurement of nanosecond time-resolved fluorescence with a directly gated interline CCD camera. Journal of Microscopy, 206(3), 233-238. https://doi.org/10.1046/j.1365-2818.2002.01030.x

• Journal Article Type: Article
• Publication Date: Jun 1, 2002
• Deposit Date: Aug 8, 2007
• Journal: Journal of Microscopy
• Print ISSN: 0022-2720
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 206
• Issue: 3
• Pages: 233-238
• DOI: https://doi.org/10.1046/j.1365-2818.2002.01030.x
• Keywords: CCD, FLIM, fluorescence lifetime imaging, gating, time-resolved detection
• Publisher URL: http://dx.doi.org/10.1046/j.1365-2818.2002.01030.x