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Measurement of nanosecond time-resolved fluorescence with a directly gated interline CCD camera

Mitchell, A; Wall, JE; Murray, JG; Morgan, CG

Authors

A Mitchell

JE Wall

JG Murray

CG Morgan



Abstract

CCD cameras coupled optically to gated image intensifiers have been used for fast time-resolved measurements for some years. Image intensifiers have disadvantages, however, and for some applications it would be better if the image sensor could be gated directly at high speed. Control of the 'charge drain' function on an interline-transfer CCD allows the sensor to be switched rapidly from an insensitive state. The temporal and spatial properties of the charge drain are explored in the present paper and it is shown that nanosecond time resolution with acceptable spatial uniformity can be achieved for a small commercial sensor. A fluorescence lifetime imaging system is demonstrated, based on a repetitively pulsed laser excitation source synchronized to the CCD control circuitry via a programmable delay unit.

Citation

Mitchell, A., Wall, J., Murray, J., & Morgan, C. (2002). Measurement of nanosecond time-resolved fluorescence with a directly gated interline CCD camera. Journal of Microscopy, 206(3), 233-238. https://doi.org/10.1046/j.1365-2818.2002.01030.x

Journal Article Type Article
Publication Date Jun 1, 2002
Deposit Date Aug 8, 2007
Journal Journal of Microscopy
Print ISSN 0022-2720
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 206
Issue 3
Pages 233-238
DOI https://doi.org/10.1046/j.1365-2818.2002.01030.x
Keywords CCD, FLIM, fluorescence lifetime imaging, gating, time-resolved detection
Publisher URL http://dx.doi.org/10.1046/j.1365-2818.2002.01030.x