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Electrochemical detection of asparaginase antibodies using bifunctionalized carbon interfaces

Marshall, Daisy; Lehr, Joshua

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Authors

Daisy Marshall



Abstract

The early detection of anti-asparaginase biomarker can facilitate timely modification of asparaginase chemotherapy, thereby avoiding serious complications. Herein we describe the preparation of a novel electrochemical biosensing interface for rapid detection of anti-asparaginase in the picomolar range (1–10000 pM). Coimmobilization of ferrocene and asparaginase on a carbon interface (via diazonium grafting) facilitates transduction through attenuation of the surface-bound ferrocene redox couple. The limit of detection of 0.8 pM for this point-of-care applicable method compares favourably to that of traditional faradaic assaying (2.0 pM) where transduction occurs by the target blocking the diffusion of the solution redox probe [Fe(CN)6]3-/4-.

Citation

Marshall, D., & Lehr, J. (in press). Electrochemical detection of asparaginase antibodies using bifunctionalized carbon interfaces. Electroanalysis, https://doi.org/10.1002/elan.202200456

Journal Article Type Article
Acceptance Date Jan 20, 2023
Online Publication Date Feb 10, 2023
Deposit Date Feb 13, 2023
Publicly Available Date Feb 14, 2023
Journal Electroanalysis
Print ISSN 1040-0397
Electronic ISSN 1521-4109
Publisher Wiley
DOI https://doi.org/10.1002/elan.202200456
Keywords Electrochemistry, Analytical Chemistry
Publisher URL https://doi.org/10.1002/elan.202200456
Additional Information Funders : Kidscan Cancer Research Charity;UK Research & Innovation (UKRI);Engineering & Physical Sciences Research Council (EPSRC)
Grant Number: 1094946
Grant Number: EP/R00661X/1
Grant Number: EP/P025021/1
Grant Number: EP/P025498/1

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