Electrochemical detection of asparaginase antibodies using bifunctionalized carbon interfaces

Marshall, Daisy; Lehr, Joshua

Abstract

The early detection of anti-asparaginase biomarker can facilitate timely modification of asparaginase chemotherapy, thereby avoiding serious complications. Herein we describe the preparation of a novel electrochemical biosensing interface for rapid detection of anti-asparaginase in the picomolar range (1–10000 pM). Coimmobilization of ferrocene and asparaginase on a carbon interface (via diazonium grafting) facilitates transduction through attenuation of the surface-bound ferrocene redox couple. The limit of detection of 0.8 pM for this point-of-care applicable method compares favourably to that of traditional faradaic assaying (2.0 pM) where transduction occurs by the target blocking the diffusion of the solution redox probe [Fe(CN)6]3-/4-.

Citation

Marshall, D., & Lehr, J. (in press). Electrochemical detection of asparaginase antibodies using bifunctionalized carbon interfaces. Electroanalysis, https://doi.org/10.1002/elan.202200456

• Journal Article Type: Article
• Acceptance Date: Jan 20, 2023
• Online Publication Date: Feb 10, 2023
• Deposit Date: Feb 13, 2023
• Publicly Available Date: Feb 14, 2023
• Journal: Electroanalysis
• Print ISSN: 1040-0397
• Electronic ISSN: 1521-4109
• Publisher: Wiley
• DOI: https://doi.org/10.1002/elan.202200456
• Keywords: Electrochemistry, Analytical Chemistry
• Publisher URL: https://doi.org/10.1002/elan.202200456
• Additional Information: Funders : Kidscan Cancer Research Charity;UK Research & Innovation (UKRI);Engineering & Physical Sciences Research Council (EPSRC); Grant Number: 1094946; Grant Number: EP/R00661X/1; Grant Number: EP/P025021/1; Grant Number: EP/P025498/1