Dr Alejandra Diaz De Rienzo M.A.DiazDeRienzo@salford.ac.uk
Lecturer in Biomedicine
Bio surfactant Production in Aerobic and Anaerobic Conditions by Different Species of the Genus Pseudomonas
Diaz De Rienzo, MA; Urdaneta, I; Dorta, B
Authors
I Urdaneta
B Dorta
Abstract
In a time where surface active agents are capable of reducing the energy of the bonds between water molecules by interacting with them to reduce surface tension, it would be unwise not to be able to generate these in masses. Different Pseudomonas species were grown in MSP (minimal sulphate phosphate) media containing salts, glycerol and glucose. P. aeruginosa grown aerobically in the presence of glycerol as carbon source showed the highest emulsion percentage (81.48%), most significant decrease in surface tension (20 mN/m) and rhamnose production of 2.86 mg/mL. However, in anaerobic conditions there was no emulsion, rhamnolipid production or decrease in surface tension. The rhamnolipids were molecularly characterized using ESI-MS (electrospray ionization-mass spectrometry), P. aeruginosa CVCM 411 is able to produce mono-rhamnolipids and di-rhamnolipids, being rhamnolipid RhC10C12:1 the predominant monomer. The specific growth rate for isolates of P. aeruginosa and P. fluorescens in MSP are 0.6732 h-1 and 0.2181 h-1, respectively. In conclusion, the formation of rhamnolipids by P. aeruginosa is linked to its growth (depending on μ), and its ability to generate about 35% of the μmax, in the presence of glucose (carbon source) and glycerol (applied as pulses).
Citation
Diaz De Rienzo, M., Urdaneta, I., & Dorta, B. (2014). Bio surfactant Production in Aerobic and Anaerobic Conditions by Different Species of the Genus Pseudomonas. Journal of Life Sciences, 8(3), 201-210
Journal Article Type | Article |
---|---|
Publication Date | Mar 30, 2014 |
Deposit Date | Aug 24, 2023 |
Journal | Journal of Life Sciences |
Print ISSN | 1934-7391 |
Publisher | David Publishing |
Peer Reviewed | Peer Reviewed |
Volume | 8 |
Issue | 3 |
Pages | 201-210 |
Related Public URLs | https://researchonline.ljmu.ac.uk/id/eprint/7231/ |
You might also like
Surfactants: physicochemical interactions with biological macromolecules.
(2021)
Journal Article
The effect of sophorolipids against microbial biofilms on medical-grade silicone
(2020)
Journal Article
Downloadable Citations
About USIR
Administrator e-mail: library-research@salford.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search