Dr Matthew Jones M.A.Jones9@salford.ac.uk
Lecturer
Dr Matthew Jones M.A.Jones9@salford.ac.uk
Lecturer
James Hanison
Renata Apreutesei
Basmah Allarakia
Dr Sara Namvar S.Namvar@salford.ac.uk
Associate Professor/Reader
Deepa Shruthi Ramaswamy
Daniel Horner
Lucy Smyth
Richard Body
Malachy Columb
Mahesan Nirmalan
Prof Niroshini Nirmalan N.J.Nirmalan@salford.ac.uk
Interim Director of HNS
Background and objectives: There is a need to develop objective risk stratification tools to define efficient care pathways for trauma patients. Biomarker-based point of care testing may strengthen existing clinical tools currently available for this purpose. The dysregulation of pro- and anti-inflammatory cytokines in the pathogenesis of organ failure is well recognised. This study was carried out to evaluate whether blood concentrations of IL-6, IL-10, and IL-6:IL-10 ratios in the early stages of the illness are significantly different in patients with worsening organ function. Materials and methods: In this prospective observational cohort study, plasma concentrations of IL-6 and IL-10 on days 1, 3 and 5 were measured in 91 major trauma patients using a multiplexed cytometric bead array approach. A composite measure of adverse outcome - defined as SOFA ≥ 2 or mortality at 7 days, was the primary outcome. IL-6 and IL-10 concentrations in early samples (days 1, 3 & 5) in patients who developed SOFA ≥ 2 on day 7 were compared against those who did not. Similar composite outcome groups at day 5 and in groups with worsening or improving SOFA scores (ΔSOFA) at days 7 and 5 were undertaken as secondary analyses. Results: Stratification on day 7, 44 (48%) patients showed adverse outcomes. These adverse outcomes associated with significantly greater IL-6 concentrations on days 1 and 5 (Day 1: 47.65 [23.24-78.68] Vs 73.69 [39.93 – 118.07] pg/mL, P = 0.040 and Day 5: 12.85 [5.80-19.51] Vs 28.90 [8.78-74.08] pg/mL; P = 0.0019). Similarly, IL-10 levels were significantly greater in the adverse outcome group on days 3 and 5 (Day 3: 2.54 [1.76-3.19] Vs 3.16 [2.68-4.21] pg/mL; P = 0.044 and Day 5: 2.03 [1.65-2.55] Vs 2.90 [2.00-5.06] pg/mL; P <0.001). IL-6 and IL-10 concentrations were also significantly elevated in the adverse outcome groups at day 3 and day 5 when stratified on day 5 outcomes. Both IL-6 and IL-6:IL-10 were found to be significantly elevated on days 1 and 3 when stratified based on ΔSOFA at day 5. This significance was lost when stratified on day 7 scores. Conclusions: Early IL-6 and IL-10 concentrations are significantly greater in patients who develop worsening organ functions downstream. These differences may provide an alternate biomarker-based approach to strengthen risk stratification in trauma patients.
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 8, 2023 |
Online Publication Date | Nov 23, 2023 |
Publication Date | Nov 23, 2023 |
Deposit Date | Nov 23, 2023 |
Publicly Available Date | Nov 27, 2023 |
Journal | Frontiers in Immunology |
Electronic ISSN | 1664-3224 |
Publisher | Frontiers Media |
Peer Reviewed | Peer Reviewed |
Volume | 14 |
Pages | 1276171 |
DOI | https://doi.org/10.3389/fimmu.2023.1276171 |
Keywords | trauma, cytokines, biomarkers, SOFA, interleukin-6, interleukin-10 |
Publisher URL | https://www.frontiersin.org/journals/immunology |
Published Version
(4 Mb)
PDF
Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
Authentic Pathology Specimen Reception: A Valuable Resource for Developing Biomedical Science Student Competencies and Employability
(2024)
Presentation / Conference
The impact of proctored online assessments on academic attainment, student experience and wellbeing
(2023)
Presentation / Conference
Aspergillus fumigatus—Host Interactions Mediating Airway Wall Remodelling in Asthma
(2022)
Journal Article
About USIR
Administrator e-mail: library-research@salford.ac.uk
This application uses the following open-source libraries:
Apache License Version 2.0 (http://www.apache.org/licenses/)
Apache License Version 2.0 (http://www.apache.org/licenses/)
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2025
Advanced Search