Visual pigment evolution in Characiformes: The dynamic interplay of teleost whole‐genome duplication, surviving opsins and spectral tuning

Escobar‐Camacho, Daniel; Carleton, Karen; Narain, Devika; Pierotti, Michele

Abstract

Vision represents an excellent model for studying adaptation, given the genotype-to-phenotype map that has been characterized in a number of taxa. Fish possess a diverse range of visual sensitivities and adaptations to underwater light, making them an excellent group to study visual system evolution. In particular, some speciose but understudied lineages can provide a unique opportunity to better understand aspects of visual system evolution such as opsin gene duplication and neofunctionalization. In this study, we showcase the visual system evolution of neotropical Characiformes and the spectral tuning mechanisms they exhibit to modulate their visual sensitivities. Such mechanisms include gene duplications and losses, gene conversion, opsin amino acid sequence and expression variation, and A1/A2-chromophore shifts. The Characiforms we studied utilize three cone opsin classes (SWS2, RH2, LWS) and a rod opsin (RH1). However, the characiform's entire opsin gene repertoire is a product of dynamic evolution by opsin gene loss (SWS1, RH2) and duplication (LWS, RH1). The LWS- and RH1-duplicates originated from a teleost specific whole-genome duplication as well as characiform-specific duplication events. Both LWS-opsins exhibit gene conversion and, through substitutions in key tuning sites, one of the LWS-paralogues has acquired spectral sensitivity to green light. These sequence changes suggest reversion and parallel evolution of key tuning sites. Furthermore, characiforms' colour vision is based on the expression of both LWS-paralogues and SWS2. Finally, we found interspecific and intraspecific variation in A1/A2-chromophores proportions, correlating with the light environment. These multiple mechanisms may be a result of the diverse visual environments where Characiformes have evolved.

Citation

Escobar‐Camacho, D., Carleton, K., Narain, D., & Pierotti, M. (2020). Visual pigment evolution in Characiformes: The dynamic interplay of teleost whole‐genome duplication, surviving opsins and spectral tuning. Molecular Ecology, 29(12), 2234-2253. https://doi.org/10.1111/mec.15474

• Journal Article Type: Article
• Online Publication Date: May 18, 2020
• Publication Date: 2020-06
• Deposit Date: Jan 12, 2024
• Journal: Molecular Ecology
• Print ISSN: 0962-1083
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 29
• Issue: 12
• Pages: 2234-2253
• DOI: https://doi.org/10.1111/mec.15474