Winogradsky Column Experiment: Setup presentation and methods

Morgan, Nicky

Abstract

Introducing microbial ecosystems to university students can be a difficult topic without visual aids. Winogradsky columns have been used extensively to demonstrate microbial diversity in soil environments. The soil provides a wide variety of nutrients for the microorganisms to flourish, creating microsites of specific microorganisms depending on energy requirements, carbon sources and environmental factors. This creates visible layers in the soil that can be observed to show anaerobic and aerobic changes

Instructions

The bottle will be used as a vessel for your microbes over the next 4 – 8 weeks, so ensure the bottle is leak proof before adding anything

Firstly, you will need to collect soil, this could be from a garden or lake but please ask permission before digging up somebody’s prized roses

Whilst out and about see if you can collect some water too, this could be as simple as leaving a cup outside to fill with rain water

Draw a line on your bottle at about ¼ from the top and then another line at about ¼ from the bottom.

Cut the newspaper into small pieces.

In a small mixing bowl, add the egg yolk (raw or hard-boiled) and newspaper and a small amount of mud, at least as much to fill the bottle ¼ of the way. If you are including additional ingredients, add them to this mixture.

Fill the bottle ¼ way with the mud, egg yolk, newspaper mixture.

Add mud to the top line.

Add water almost to the top. Leave a small amount of space.

Cover the column with plastic wrap and a rubber band (do not put a tight lid on because it may explode due to gases released by the microbes).

Set it in the sunlight or under a lamp.

Let your column grow and watch for changes over the next 4 to 8 weeks. Take pictures of the changes

• Other Type: Teaching Resource
• Acceptance Date: Mar 24, 2025
• Online Publication Date: Mar 24, 2025
• Publication Date: Mar 24, 2025
• Deposit Date: Mar 31, 2025
• DOI: https://doi.org/10.25416/NTR.28631474.v1