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All Outputs (26)

Celastrol induces pro-apoptotic and anti-migratory effects against medulloblastoma subtypes (2025)
Presentation / Conference Contribution

Introduction
Medulloblastoma accounts for 20-25% of all paediatric brain tumours diagnosed worldwide (de Medeiros et al., 2019). Current treatment regimens are effective. However, most options lead to long-term off-target effects. Therefore, the con... Read More about Celastrol induces pro-apoptotic and anti-migratory effects against medulloblastoma subtypes.

The effects of doxorubicin on calcium handling and contractility in sheep ventricular myocytes; role of oxidative stress (2023)
Presentation / Conference Contribution

Anthracyclines such as doxorubicin (DOX) are effective chemotherapeutics and contribute to improved cancer survival rates in children and adults. However, anthracyclines exhibit acute and chronic cardiotoxicity which can produce heart failure in canc... Read More about The effects of doxorubicin on calcium handling and contractility in sheep ventricular myocytes; role of oxidative stress.

The effects of tumour necrosis factor-alpha and interleukin-1 beta on cardiac intracellular calcium handling (2018)
Presentation / Conference Contribution

Cytokines including tumour necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) are known to mediate systolic and diastolic myocardial dysfunction in inflammatory disease such as sepsis. To provide a cellular basis, we separately studied the... Read More about The effects of tumour necrosis factor-alpha and interleukin-1 beta on cardiac intracellular calcium handling.

The effects of TBQ on cardiac intracellular ATP levels; role of oxidative phosphorylation and oxidative stress (2017)
Presentation / Conference Contribution

In a recent study, 2,5-Di-(tert-butyl)−1,4-benzohydroquinone (TBQ) produced a concentration dependent and fully reversible inhibition of the sarcoplasmic reticulum Ca2+ ATPase (SERCA) in rat ventricular myocytes1. While TBQ is a potentially useful re... Read More about The effects of TBQ on cardiac intracellular ATP levels; role of oxidative phosphorylation and oxidative stress.

Redox Control of Protein Kinase G Iα Fine-Tunes the Frank-Starling Law of the Heart in Vivo by Regulating Diastolic Relaxation (2016)
Presentation / Conference Contribution

The Frank-Starling law describes a regulatory mechanism that allows the amount of blood that enters the heart to be precisely matched with the amount that is pumped out to the arterial circulation, independently of external regulation. Mechanisms tha... Read More about Redox Control of Protein Kinase G Iα Fine-Tunes the Frank-Starling Law of the Heart in Vivo by Regulating Diastolic Relaxation.

The effects of 2′,5′-di(tert-butyl)-1,4-benzohydroquinone (TBQ) on calcium handling in rat ventricular myocytes (2014)
Presentation / Conference Contribution

2′,5′-di(tert-butyl)-1,4-benzohydroquinone (TBQ) is a reversible inhibitor of SERCA, potentially making it a useful tool to study the effects of SERCA inhibition. It has been used in a variety of cell types including ventricular myocytes (1). However... Read More about The effects of 2′,5′-di(tert-butyl)-1,4-benzohydroquinone (TBQ) on calcium handling in rat ventricular myocytes.

Simultaneous measurement of cytoplasmic and SR calcium during modulation of ryanodine receptor open probability in canine ventricular myocytes (2013)
Presentation / Conference Contribution

We have previously shown in rat ventricular myocytes that modulation of ryanodine receptor (RyR) open probability using low concentrations of caffeine has no maintained effect on the amplitude of systolic Ca (Trafford et al, 2000). We suggested that... Read More about Simultaneous measurement of cytoplasmic and SR calcium during modulation of ryanodine receptor open probability in canine ventricular myocytes.

Simultaneous Measurement of Cytoplasmic and SR Calcium during Modulation of Ryanodine Receptor Open Probability in Dog Ventricular Myocytes (2013)
Presentation / Conference Contribution

We have previously found that, in rat ventricular myocytes, increasing ryanodine receptor open probability using low concentrations of caffeine had no maintained effect on the amplitude of systolic Ca. On application of caffeine, following an initial... Read More about Simultaneous Measurement of Cytoplasmic and SR Calcium during Modulation of Ryanodine Receptor Open Probability in Dog Ventricular Myocytes.

Modulation of ryanodine receptor open probability has no maintained effect on the amplitude of systolic calcium release in sheep or dog ventricular myocytes (2012)
Presentation / Conference Contribution

Caffeine is frequently used as an agent to increase the open probability of the Ryanodine receptor (RyR). However, our group has previously demonstrated that in rat, application of 500 µM caffeine has no sustained effect on systolic Ca. Rather, follo... Read More about Modulation of ryanodine receptor open probability has no maintained effect on the amplitude of systolic calcium release in sheep or dog ventricular myocytes.

In cardiac ventricular myocytes correction of severe leak from the sarcoplasmic reticulum increases the amplitude and the rate of decay of the systolic calcium transient (2012)
Presentation / Conference Contribution

INTRODUCTION: In cardiac muscle the amplitude and duration of the systolic calcium (Ca) transient and thence contraction are modulated by the release and re-uptake of Ca from the sarcoplasmic reticulum (SR). In heart failure there is a decrease in bo... Read More about In cardiac ventricular myocytes correction of severe leak from the sarcoplasmic reticulum increases the amplitude and the rate of decay of the systolic calcium transient.

Measurement of intracellular sodium following a change of pacing frequency in sheep ventricular myocytes (2012)
Presentation / Conference Contribution

Changes in action potential duration (APD) occur following a change in heart rate. The time course of these changes has two distinct initial phases; i) a rapid component occurring immediately upon a change of rate and, ii) a slower component occurrin... Read More about Measurement of intracellular sodium following a change of pacing frequency in sheep ventricular myocytes.

Increased ICaL Underlies Ventricular Action-Potential Prolongation After Spontaneous Ca2+ Release From The Sarcoplasmic Reticulum (2011)
Presentation / Conference Contribution

Background: Spontaneous Ca2+ release from the sarcoplasmic reticulum (SCR) can cause delayed afterdepolarizations (DADs) and triggered activity, contributing to arrhythmogenesis during Ca2+ overload. Excessive beat-to-beat variability of repolarizati... Read More about Increased ICaL Underlies Ventricular Action-Potential Prolongation After Spontaneous Ca2+ Release From The Sarcoplasmic Reticulum.

Changes of action potential duration and intracellular calcium following a change of pacing frequency in sheep ventricular myocytes (2011)
Presentation / Conference Contribution

Action potential duration (APD) decreases following an increase in heart rate and vice versa. Changes in APD can occur with a time course of hours to days, a phenomenon known as cardiac memory. In the short term however, changes occur both rapidly (w... Read More about Changes of action potential duration and intracellular calcium following a change of pacing frequency in sheep ventricular myocytes.