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Synthesis and photophysical properties of meso‐aminophenyl-substituted heptamethine dyes as potential leads to new contrast agents (2019)
Journal Article
Okoh, O., Critchley, M., Bisby, R., Lawrence, C., Wainwright, M., & Smith, R. (2019). Synthesis and photophysical properties of meso‐aminophenyl-substituted heptamethine dyes as potential leads to new contrast agents. Coloration Technology, 135(4), 305-311. https://doi.org/10.1111/cote.12403

The series of rigid meso‐aminophenyl‐substituted heptamethine dyes presented herein exhibit surprising fluorescence properties, demonstrating larger Stokes shifts when compared with both structurally similar rigid meso‐chlorophenyl and linear heptame... Read More about Synthesis and photophysical properties of meso‐aminophenyl-substituted heptamethine dyes as potential leads to new contrast agents.

Modulation of antimalarial activity at a putative bisquinoline receptor in vivo using fluorinated bisquinolines (2017)
Journal Article
Fielding, A., Lukinović, V., Evans, P., Alizadeh-Shekalgourabi, S., Bisby, R., Drew, M., …Ismail, F. (2017). Modulation of antimalarial activity at a putative bisquinoline receptor in vivo using fluorinated bisquinolines. Chemistry - A European Journal, 23(28), 6811-6828. https://doi.org/10.1002/chem.201605099

Antimalarials can interact with heme covalently, by π⋅⋅⋅π interactions or by hydrogen bonding. Consequently, the prototropy of 4‐aminoquinolines and quinoline methanols was investigated by using quantum mechanics. Calculations showed mefloquine proto... Read More about Modulation of antimalarial activity at a putative bisquinoline receptor in vivo using fluorinated bisquinolines.

New Approaches to Photodynamic Therapy from Type I, II and III to Type IV Using One or More Photons (2017)
Journal Article
Scherer, K., Bisby, R., Botchway, S., & Parker, A. (2017). New Approaches to Photodynamic Therapy from Type I, II and III to Type IV Using One or More Photons. Anti-Cancer Agents in Medicinal Chemistry, 17(2), 171-189. https://doi.org/10.2174/1871520616666160513131723

Photodynamic therapy (PDT) is an alternative cancer treatment to conventional surgery, radiotherapy and chemotherapy. It is based on activating a drug with light that triggers the generation of cytotoxic species that promote tumour cell killing. At p... Read More about New Approaches to Photodynamic Therapy from Type I, II and III to Type IV Using One or More Photons.

Three-dimensional imaging and uptake of the anticancer drug combretastatin in cell spheroids and photoisomerization in gels with multiphoton excitation (2015)
Journal Article
Scherer, K., Bisby, R., Botchway, S., Hadfield, J., Haycock, J., & Parker, A. (2015). Three-dimensional imaging and uptake of the anticancer drug combretastatin in cell spheroids and photoisomerization in gels with multiphoton excitation. Journal of Biomedical Optics, 20(7), 78003. https://doi.org/10.1117/1.JBO.20.7.078003

The uptake of E-combretastatins, potential pro-drugs of the anticancer Z-isomers, into multicellular spheroids has been imaged by intrinsic fluorescence in three dimensions using two-photon excited fluorescence lifetime imaging with 625 nm ultrafast... Read More about Three-dimensional imaging and uptake of the anticancer drug combretastatin in cell spheroids and photoisomerization in gels with multiphoton excitation.

A series of flexible design adaptations to the Nikon E-C1 and E-C2 confocal microscope systems for UV, multiphoton and FLIM imaging (2015)
Journal Article
Botchway, S., Scherer, K., Hook, S., Stubbs, C., Weston, E., Bisby, R., & Parker, A. (2015). A series of flexible design adaptations to the Nikon E-C1 and E-C2 confocal microscope systems for UV, multiphoton and FLIM imaging. Journal of Microscopy, 258(1), 68-78. https://doi.org/10.1111/jmi.12218

Multiphoton microscopy in combination with Fluorescence Lifetime Imaging Microscopy (FLIM) is now a key technique in biomedical and life-science research for live cell and whole animal imaging providing several advantages over the one photon confocal... Read More about A series of flexible design adaptations to the Nikon E-C1 and E-C2 confocal microscope systems for UV, multiphoton and FLIM imaging.

Anticancer phototherapy using activation of E-combretastatins by two-photon–induced isomerization (2014)
Journal Article
Hadfield, J., Bisby, R., Scherer, K., Botchway, S., & Parker, A. (2014). Anticancer phototherapy using activation of E-combretastatins by two-photon–induced isomerization. Journal of Biomedical Optics, 20(5), 1004-1. https://doi.org/10.1117/1.JBO.20.5.051004

The photoisomerization of relatively nontoxic E-combretastatins to clinically active Z-isomers is shown to occur in solution through both one- and two-photon excitations at 340 and 625 nm, respectively. The photoisomerization is also demonstrated t... Read More about Anticancer phototherapy using activation of E-combretastatins by two-photon–induced isomerization.

Ultrafast vibrational spectroscopic Studies on the photoionization of the α-Tocopherol analogue Trolox C (2014)
Journal Article
Parker, A., Bisby, R., Greetham, G., Kukura, P., Scherer, K., & Towrie, M. (2014). Ultrafast vibrational spectroscopic Studies on the photoionization of the α-Tocopherol analogue Trolox C. Journal of Physical Chemistry B, 118(42), 12087-12097. https://doi.org/10.1021/jp505113k

The initial events after photoexcitation and photoionization of α-tocopherol (vitamin E) and the analogue Trolox C have been studied by femtosecond stimulated Raman spectroscopy, transient absorption spectroscopy and time-resolved infrared spectrosco... Read More about Ultrafast vibrational spectroscopic Studies on the photoionization of the α-Tocopherol analogue Trolox C.

Spectroscopy and fluorescence lifetime imaging in live cells of a cyano-substituted combretastatin (2014)
Journal Article
combretastatin. Biomedical Spectroscopy and Imaging, 3, 211-218. https://doi.org/10.3233/BSI-140067

Fluorescence lifetime imaging has been used to observe the real-time uptake in live mammalian cells of a combretastatin-type drug analogue that is a substituted stilbene with a cyano group at the bridging olefinic bond. Fluorescence spectra in a ran... Read More about Spectroscopy and fluorescence lifetime imaging in live cells of a cyano-substituted combretastatin.

Promising near-infrared non-targeted probes: benzothiazole heptamethine cyanine dyes (2014)
Journal Article
Okoh, O., Bisby, R., Lawrence, C., Rolph, C., & Smith, R. (2014). Promising near-infrared non-targeted probes: benzothiazole heptamethine cyanine dyes. Journal of Sulfur Chemistry, 35(1), 42-56. https://doi.org/10.1080/17415993.2013.778258

A series of benzothiazole heptamethine cyanine dyes have been synthesized and their photophysical properties evaluated in relation to their structural features. These have been compared against two classical probes of this type: Indocyanine Green (IG... Read More about Promising near-infrared non-targeted probes: benzothiazole heptamethine cyanine dyes.

Time-resolved nanosecond fluorescence lifetime imaging and picosecond infrared spectroscopy of combretastatin A-4 in solution and in cellular systems (2012)
Journal Article
Bisby, R., Botchway, S., Greetham, G., Hadfield, J., Mcgown, A., Parker, A., …Towrie, M. (2012). Time-resolved nanosecond fluorescence lifetime imaging and picosecond infrared spectroscopy of combretastatin A-4 in solution and in cellular systems. Measurement Science and Technology, 23, 084001. https://doi.org/10.1088/0957-0233/23/8/084001

Fluorescence lifetime images of intrinsic fluorescence obtained with two-photon excitation at 630 nm are shown following uptake of a series of E-combretastatins into live cells, including human umbilical vein endothelial cells (HUVECs) that are the t... Read More about Time-resolved nanosecond fluorescence lifetime imaging and picosecond infrared spectroscopy of combretastatin A-4 in solution and in cellular systems.

Fluorescence lifetime imaging of propranolol uptake in living glial C6 cells (2012)
Journal Article
Bisby, R., Botchway, S., Crisostomo, A., Parker, A., & Scherer, K. (2012). Fluorescence lifetime imaging of propranolol uptake in living glial C6 cells. Journal of Spectroscopy, 27(5-6), 533-540. https://doi.org/10.1155/2012/518353

Uptake of the β-blocker drug propranolol by living glial C6 cells has been observed using fluorescence lifetime imaging with two-photon excitation at 630 nm. Both uptake and release of propranolol occur within minutes and are temperature dependent, b... Read More about Fluorescence lifetime imaging of propranolol uptake in living glial C6 cells.

Fluorescence lifetime imaging of E-combretastatin uptake and distribution in live mammalian cells (2011)
Journal Article
and distribution in live mammalian cells. European Journal of Cancer, 48(12), 1896-1903. https://doi.org/10.1016/j.ejca.2011.11.025

To investigate within live mammalian cells the uptake and disposition of combretastatins, fluorescence lifetime imaging was used with two-photon excitation (2PE). Combretastatin A4 (CA4) and analogues are potential anticancer drugs due to their abili... Read More about Fluorescence lifetime imaging of E-combretastatin uptake and distribution in live mammalian cells.

Interactions of the beta-blocker drug, propranolol, with detergents, beta-cyclodextrin and living cells studied using fluorescence spectroscopy and imaging (2010)
Journal Article
Bisby, R., Botchway, S., Crisostomo, A., Karolin, J., Parker, A., & Schroder, L. (2010). Interactions of the beta-blocker drug, propranolol, with detergents, beta-cyclodextrin and living cells studied using fluorescence spectroscopy and imaging. Spectroscopy (Ottawa. Print), 24(1-2), 137-142. https://doi.org/10.3233/SPE-2010-0415

Interactions of the beta-blocker drug, propranolol, with amphipathic systems have been studied using fluorescence spectroscopy. The results show a strong binding of propranolol with micelles of sodium dodecyl sulfate revealed through changes in the f... Read More about Interactions of the beta-blocker drug, propranolol, with detergents, beta-cyclodextrin and living cells studied using fluorescence spectroscopy and imaging.

Photoreactivity of biologically active compounds. XIX: Excited states and free radicals from the antimalarial drug primaquine (2009)
Journal Article
Kristensen, S., Edge, R., Tønnesen, H., Bisby, R., & Navaratnam, S. (2009). Photoreactivity of biologically active compounds. XIX: Excited states and free radicals from the antimalarial drug primaquine. Journal of Photochemistry and Photobiology B: Biology, 94(3), 147-157. https://doi.org/10.1016/j.jphotobiol.2008.11.006

The formation and reactivity of excited states and free radicals from primaquine was studied in order to evaluate the primary photochemical reaction mechanisms. The excited primaquine triplet was not detected, but is likely to be formed with a short... Read More about Photoreactivity of biologically active compounds. XIX: Excited states and free radicals from the antimalarial drug primaquine.

A pulse radiolysis study of free radicals formed by one electron oxidation of the antimalarial drug pyronaridine (2009)
Journal Article
Ismail, F., Drew, M., Navaratnam, S., & Bisby, R. (2009). A pulse radiolysis study of free radicals formed by one electron oxidation of the antimalarial drug pyronaridine. Research on Chemical Intermediates, 35(4), 363-377. https://doi.org/10.1007/s11164-009-0051-7

Free radicals from one-electron oxidation of the antimalarial drug pyronaridine have been studied by pulse radiolysis. The results show that pyronaridine is readily oxidised to an intermediate semiiminoquine radical by inorganic and organic free radi... Read More about A pulse radiolysis study of free radicals formed by one electron oxidation of the antimalarial drug pyronaridine.

Intracellular molecular imaging using multiphoton-excited microscopy (2009)
Thesis
Crisostomo, A. Intracellular molecular imaging using multiphoton-excited microscopy. (Thesis). Salford : University of Salford

Detection of the levels of serotonin (5-HT) present in biofluids may be used for detection of neurological diseases such as Alzheimer's 1 . This project seeks to develop technologies using multiphoton excitation and resultant luminescence of serot... Read More about Intracellular molecular imaging using multiphoton-excited microscopy.

Real-time cellular uptake of serotonin using fluorescence lifetime imaging with two-photon excitation (2008)
Journal Article
Botchway, S., Parker, A., Bisby, R., & Crisostomo, A. (2008). Real-time cellular uptake of serotonin using fluorescence lifetime imaging with two-photon excitation. Microscopy Research and Technique, 71(4), 267-273. https://doi.org/10.1002/jemt.20548

The real-time uptake of serotonin, a neurotransmitter, by rat leukaemia mast cell line RBL-2H3 and 5-hydroxytryptophan by Chinese hamster V79 cells has been studied by fluorescence lifetime imaging microscopy (FLIM), monitoring ultraviolet (340 nm) f... Read More about Real-time cellular uptake of serotonin using fluorescence lifetime imaging with two-photon excitation.

Effect of antioxidant potential in the oxygen radical absorption capacity (ORAC) assay (2008)
Journal Article
Bisby, R., Brooke, R., & Navaratnam, S. (2008). Effect of antioxidant potential in the oxygen radical absorption capacity (ORAC) assay. Food Chemistry, 108(3), 1002-1007. https://doi.org/10.1016/j.foodchem.2007.12.012

The "Oxygen Radical Absorption Capacity” (ORAC) assay (Ou, B., Hampsch-Woodill, M., Prior, R.L. (2001). Development and validation of an improved oxygen radical absorbance capacity assay using fluorescein as the fluorescent probe. Journal of Agricultur... Read More about Effect of antioxidant potential in the oxygen radical absorption capacity (ORAC) assay.

Generation of superoxide and singlet oxygen from α-tocopherolquinone and analogues. (2007)
Journal Article
Crisostomo, A., Moreno, R., Navaratnam, S., Wilkinson, J., & Bisby, R. (2007). Generation of superoxide and singlet oxygen from α-tocopherolquinone and analogues. Free Radical Research, 41(6), 730-737. https://doi.org/10.1080/10715760701324075

Three potential routes to generation of reactive oxygen species from a tocopherolquinone have been identified. The quinone of the water-soluble vitamin E analogue Trolox C (Trol-Q) is reduced by hydrated electron and isopropanol a hydroxyalkyl radica... Read More about Generation of superoxide and singlet oxygen from α-tocopherolquinone and analogues..

Near infrared multiphoton-induced generation and detection of hydroxyl radicals in a biochemical system (2007)
Journal Article
Botchway, S., Crisostomo, A., Parker, A., & Bisby, R. (2007). Near infrared multiphoton-induced generation and detection of hydroxyl radicals in a biochemical system. Archives of Biochemistry and Biophysics, 464(2), 314-321. https://doi.org/10.1016/j.abb.2007.04.026

Solutions of tryptophan and N-hydroxypyridine-2-thione (mercaptopyridine-N-oxide, MPNO) were irradiated at 335 nm. Formation of 5-hydroxytryptophan was inferred from increased fluorescence at 334 nm on excitation at 315 nm, conditions chosen for sele... Read More about Near infrared multiphoton-induced generation and detection of hydroxyl radicals in a biochemical system.