Dr Pika Miklavc's Outputs (26)

Fusion-activated Ca2+ entry via vesicular P2X4 receptors promotes fusion pore opening and exocytotic content release in pneumocytes (2011)
Journal Article
Miklavc, P., Mair, N., Wittekindt, O., Haller, T., Dietl, P., Felder, E., …Frick, M. (2011). Fusion-activated Ca2+ entry via vesicular P2X4 receptors promotes fusion pore opening and exocytotic content release in pneumocytes. Proceedings of the National Academy of Sciences of the United States of America, 108(35), 14503-14508. https://doi.org/10.1073/pnas.1101039108

Ca2+ is considered a key element in multiple steps during regulated exocytosis. During the postfusion phase, an elevated cytoplasmic Ca2+ concentration ([Ca2+])c leads to fusion pore dilation. In neurons and neuroendocrine cells, this results from ac... Read More about Fusion-activated Ca2+ entry via vesicular P2X4 receptors promotes fusion pore opening and exocytotic content release in pneumocytes.

Fusion-Activated Ca2+ Entry: An “Active Zone” of Elevated Ca2+ during the Postfusion Stage of Lamellar Body Exocytosis in Rat Type II Pneumocytes (2010)
Journal Article
Miklavc, P., Frick, M., Wittekindt, O., Haller, T., & Dietl, P. (2010). Fusion-Activated Ca2+ Entry: An “Active Zone” of Elevated Ca2+ during the Postfusion Stage of Lamellar Body Exocytosis in Rat Type II Pneumocytes. PLoS ONE, 5(6), e10982. https://doi.org/10.1371/journal.pone.0010982

Background

Ca2+ is essential for vesicle fusion with the plasma membrane in virtually all types of regulated exocytoses. However, in contrast to the well-known effects of a high cytoplasmic Ca2+ concentration ([Ca2+]c) in the prefusion phase, the... Read More about Fusion-Activated Ca2+ Entry: An “Active Zone” of Elevated Ca2+ during the Postfusion Stage of Lamellar Body Exocytosis in Rat Type II Pneumocytes.

Existence of exocytotic hemifusion intermediates with a lifetime of up to seconds in type II pneumocytes (2009)
Journal Article
Miklavc, P., Albrecht, S., Wittekindt, O., Schullian, P., Haller, T., & Dietl, P. (2009). Existence of exocytotic hemifusion intermediates with a lifetime of up to seconds in type II pneumocytes. Biochemical Journal, 424(1), 7-14. https://doi.org/10.1042/BJ20091094

Exocytosis proceeds through prefusion stages such as hemifusion, but hemifusion is still an elusive intermediate of unknown duration. Using darkfield and fluorescence microscopy in ATII (alveolar type II) cells containing large secretory vesicles (LB... Read More about Existence of exocytotic hemifusion intermediates with a lifetime of up to seconds in type II pneumocytes.

Ca2+‐Dependent Actin Coating of Lamellar Bodies after Exocytotic Fusion: A Prerequisite for Content Release or Kiss‐and‐Run (2009)
Journal Article
Miklavc, P., Wittekindt, O. H., Felder, E., & Dietl, P. (2009). Ca2+‐Dependent Actin Coating of Lamellar Bodies after Exocytotic Fusion: A Prerequisite for Content Release or Kiss‐and‐Run. Annals of the New York Academy of Sciences, 1152(1), 43-52. https://doi.org/10.1111/j.1749-6632.2008.03989.x

Type II pneumocytes secrete surfactant, a lipoprotein-like substance reducing the surface tension in the lung, by regulated exocytosis of secretory vesicles termed lamellar bodies (LBs). This secretory process is characterized by a protracted postfus... Read More about Ca2+‐Dependent Actin Coating of Lamellar Bodies after Exocytotic Fusion: A Prerequisite for Content Release or Kiss‐and‐Run.

Mechanical strain of alveolar type II cells in culture: changes in the transcellular cytokeratin network and adaptations (2008)
Journal Article
Felder, E., Siebenbrunner, M., Busch, T., Fois, G., Miklavc, P., Walther, P., & Dietl, P. (2008). Mechanical strain of alveolar type II cells in culture: changes in the transcellular cytokeratin network and adaptations. American journal of physiology. Lung cellular and molecular physiology, 295(5), L849-L857. https://doi.org/10.1152/ajplung.00503.2007

Mechanical forces exert multiple effects in cells, ranging from altered protein expression patterns to cell damage and death. Despite undisputable biological importance, little is known about structural changes in cells subjected to strain ex vivo. H... Read More about Mechanical strain of alveolar type II cells in culture: changes in the transcellular cytokeratin network and adaptations.

Lamellar body exocytosis by cell stretch or purinergic stimulation : possible physiological roles, messengers and mechanisms
Journal Article
Dietl, P., Liss, B., Felder, E., Miklavc, P., & Wirtz, H. Lamellar body exocytosis by cell stretch or purinergic stimulation : possible physiological roles, messengers and mechanisms. Cellular Physiology and Biochemistry, 25(001), 001-012. https://doi.org/10.1159/000272046

A major function of the pulmonary alveolar type II cell is the secretion of surfactant, a lipoprotein-like substance, via exocytosis of secretory vesicles termed lamellar bodies (LBs). The process of surfactant secretion is remarkable in several aspe... Read More about Lamellar body exocytosis by cell stretch or purinergic stimulation : possible physiological roles, messengers and mechanisms.