Outputs (62)

The effects of 2′,5′-di(tert-butyl)-1,4-benzohydroquinone (TBQ) on calcium handling in rat ventricular myocytes (2014)
Presentation / Conference Contribution

2′,5′-di(tert-butyl)-1,4-benzohydroquinone (TBQ) is a reversible inhibitor of SERCA, potentially making it a useful tool to study the effects of SERCA inhibition. It has been used in a variety of cell types including ventricular myocytes (1). However... Read More about The effects of 2′,5′-di(tert-butyl)-1,4-benzohydroquinone (TBQ) on calcium handling in rat ventricular myocytes.

Direct measurements of SR free Ca reveal the mechanism underlying the transient effects of RyR potentiation under physiological conditions (2014)
Journal Article
Greensmith, D., Galli, G., Trafford, A., & Eisner, D. (2014). Direct measurements of SR free Ca reveal the mechanism underlying the transient effects of RyR potentiation under physiological conditions. Cardiovascular Research, 103(4), 554-563. https://doi.org/10.1093/cvr/cvu158

AIMS: Most of the calcium that activates contraction is released from the sarcoplasmic reticulum (SR) through the ryanodine receptor (RyR). It is controversial whether activators of the RyR produce a maintained increase in the amplitude of the systol... Read More about Direct measurements of SR free Ca reveal the mechanism underlying the transient effects of RyR potentiation under physiological conditions.

Ca analysis : an Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis (2013)
Journal Article
Greensmith, D. (2014). Ca analysis : an Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis. Computer Methods and Programs in Biomedicine, 113(1), 241-250. https://doi.org/10.1016/j.cmpb.2013.09.004

Here I present an Excel based program for the analysis of intracellular Ca transients recorded using fluorescent indicators. The program can perform all the necessary steps which convert recorded raw voltage changes into meaningful physiological info... Read More about Ca analysis : an Excel based program for the analysis of intracellular calcium transients including multiple, simultaneous regression analysis.

The effects of tumor necrosis factor-alpha on systolic and diastolic function in rat ventricular myocytes (2013)
Journal Article
Greensmith, D., & Nirmalan, M. (2013). The effects of tumor necrosis factor-alpha on systolic and diastolic function in rat ventricular myocytes. Physiological Reports, 1(4), e00093. https://doi.org/10.1002/phy2.93

The proinflammatory cytokine tumor necrosis factor-alpha (TNF-α) is associated with myocardial dysfunction observed in sepsis and septic shock. There are two fundamental components to this dysfunction. (1) systolic dysfunction; and (2) diastolic dysf... Read More about The effects of tumor necrosis factor-alpha on systolic and diastolic function in rat ventricular myocytes.

Simultaneous measurement of cytoplasmic and SR calcium during modulation of ryanodine receptor open probability in canine ventricular myocytes (2013)
Presentation / Conference Contribution

We have previously shown in rat ventricular myocytes that modulation of ryanodine receptor (RyR) open probability using low concentrations of caffeine has no maintained effect on the amplitude of systolic Ca (Trafford et al, 2000). We suggested that... Read More about Simultaneous measurement of cytoplasmic and SR calcium during modulation of ryanodine receptor open probability in canine ventricular myocytes.

Increased sarcoplasmic reticulum diastolic calcium leak can produce biphasic decay of the cardiac systolic calcium transient (2013)
Presentation / Conference Contribution

Simultaneous Measurement of Cytoplasmic and SR Calcium during Modulation of Ryanodine Receptor Open Probability in Dog Ventricular Myocytes (2013)
Presentation / Conference Contribution

We have previously found that, in rat ventricular myocytes, increasing ryanodine receptor open probability using low concentrations of caffeine had no maintained effect on the amplitude of systolic Ca. On application of caffeine, following an initial... Read More about Simultaneous Measurement of Cytoplasmic and SR Calcium during Modulation of Ryanodine Receptor Open Probability in Dog Ventricular Myocytes.

Diastolic spontaneous calcium release from the sarcoplasmic reticulum increases beat-to-beat variability of repolarization in canine ventricular myocytes after β-adrenergic stimulation (2013)
Journal Article

RATIONALE

Spontaneous Ca(2+) release (SCR) from the sarcoplasmic reticulum can cause delayed afterdepolarizations and triggered activity, contributing to arrhythmogenesis during β-adrenergic stimulation. Excessive beat-to-beat variability of repol... Read More about Diastolic spontaneous calcium release from the sarcoplasmic reticulum increases beat-to-beat variability of repolarization in canine ventricular myocytes after β-adrenergic stimulation.

Modulation of ryanodine receptor open probability has no maintained effect on the amplitude of systolic calcium release in sheep or dog ventricular myocytes (2012)
Presentation / Conference Contribution

Caffeine is frequently used as an agent to increase the open probability of the Ryanodine receptor (RyR). However, our group has previously demonstrated that in rat, application of 500 µM caffeine has no sustained effect on systolic Ca. Rather, follo... Read More about Modulation of ryanodine receptor open probability has no maintained effect on the amplitude of systolic calcium release in sheep or dog ventricular myocytes.

In cardiac ventricular myocytes correction of severe leak from the sarcoplasmic reticulum increases the amplitude and the rate of decay of the systolic calcium transient (2012)
Presentation / Conference Contribution

INTRODUCTION: In cardiac muscle the amplitude and duration of the systolic calcium (Ca) transient and thence contraction are modulated by the release and re-uptake of Ca from the sarcoplasmic reticulum (SR). In heart failure there is a decrease in bo... Read More about In cardiac ventricular myocytes correction of severe leak from the sarcoplasmic reticulum increases the amplitude and the rate of decay of the systolic calcium transient.