Outputs (29)

Enhanced Activity and Stability of an Acetyl Xylan Esterase in Hydrophilic Alcohols through Site-Directed Mutagenesis (2023)
Journal Article
Madubuike, H., & Ferry, N. (in press). Enhanced Activity and Stability of an Acetyl Xylan Esterase in Hydrophilic Alcohols through Site-Directed Mutagenesis. Molecules, 28(21), 7393. https://doi.org/10.3390/molecules28217393

Current demands for the development of suitable biocatalysts showing high process performance is stimulated by the need to replace current chemical synthesis with cleaner alternatives. A drawback to the use of biocatalysts for unique applications is... Read More about Enhanced Activity and Stability of an Acetyl Xylan Esterase in Hydrophilic Alcohols through Site-Directed Mutagenesis.

Characterisation of a Novel Acetyl Xylan Esterase (BaAXE) screened from the Gut Microbiota of the Common Black Slug (Arion ater) (2022)
Journal Article
Madubuike, H., & Ferry, N. (2022). Characterisation of a Novel Acetyl Xylan Esterase (BaAXE) screened from the Gut Microbiota of the Common Black Slug (Arion ater). Molecules, 27(9), 2999. https://doi.org/10.3390/molecules27092999

Acetyl xylan esterases (AXEs) are enzymes capable of hydrolysing the acetyl bonds in acetylated xylan, allowing for enhanced activity of backbone-depolymerizing enzymes. Bioprospecting novel AXE is essential in designing enzyme cocktails with desired... Read More about Characterisation of a Novel Acetyl Xylan Esterase (BaAXE) screened from the Gut Microbiota of the Common Black Slug (Arion ater).

Fungal microbiomes are determined by host phylogeny and exhibit widespread associations with the bacterial microbiome (2021)
Journal Article
Harrison, X., McDevitt, A., Dunn, J., Grifftihs, S., Benvenuto, C., Birtles, R., …Antwis, R. (2021). Fungal microbiomes are determined by host phylogeny and exhibit widespread associations with the bacterial microbiome. Proceedings of the Royal Society B: Biological Sciences, 288(1957), 20210552. https://doi.org/10.1098/rspb.2021.0552

Interactions between hosts and their resident microbial communities are a fundamental component of fitness for both agents. Though recent research has highlighted the importance of interactions between animals and their bacterial communities, compara... Read More about Fungal microbiomes are determined by host phylogeny and exhibit widespread associations with the bacterial microbiome.

Characterization of sialic acid affinity of the binding domain of mistletoe lectin isoform one (2021)
Journal Article
Mohammed, S., & Ferry, N. (2021). Characterization of sialic acid affinity of the binding domain of mistletoe lectin isoform one. International Journal of Molecular Sciences, 22(15), e8284. https://doi.org/10.3390/ijms22158284

Sialic acid (Sia) is considered as one of the most important biomolecules of life since its derivatives and terminal orientations on cell membranes and macromolecules play a major role in many biological and pathological processes. To date, there is... Read More about Characterization of sialic acid affinity of the binding domain of mistletoe lectin isoform one.

Circular and linear : a tale of aptamer selection for the activation of SIRT1 to induce death in cancer cells (2020)
Journal Article
Al-Sudani, B., Ragazzon-Smith, A., Aziz, A., Alansari, R., Ferry, N., Krstic- Demonacos, M., & Ragazzon, P. (2020). Circular and linear : a tale of aptamer selection for the activation of SIRT1 to induce death in cancer cells. RSC Advances, 10(73), 45008-45018. https://doi.org/10.1039/D0RA07857C

It is a challenge to select the right target to treat conditions without affecting non-diseased cells. Cancer belongs to the top 10 causes of death in the world and it remains difficult to treat. Amongst cancer emerging targets, silent information re... Read More about Circular and linear : a tale of aptamer selection for the activation of SIRT1 to induce death in cancer cells.

Metagenomic analysis of the gut microbiome of the common black slug Arion ater in search of novel lignocellulose degrading enzymes (2017)
Journal Article
Joynson, R., Pritchard, L., Ekenakema, O., & Ferry, N. (2017). Metagenomic analysis of the gut microbiome of the common black slug Arion ater in search of novel lignocellulose degrading enzymes. Frontiers in Microbiology, 8, 2181. https://doi.org/10.3389/fmicb.2017.02181

Some eukaryotes are able to gain access to well-protected carbon sources in plant biomass by exploiting microorganisms in the environment or harbored in their digestive system. One is the land pulmonate Arion ater, which takes advantage of a gut micr... Read More about Metagenomic analysis of the gut microbiome of the common black slug Arion ater in search of novel lignocellulose degrading enzymes.

Proteomic analysis shows that stress response proteins are significantly up-regulated in resistant diploid wheat (Triticum monococcum) in response to attack by the grain aphid (Sitobion avenae) (2015)
Journal Article
Guan, W., Ferry, N., Edwards, M., Bell, H., Othman, H., Gatehouse, J., & Gatehouse, A. (2015). Proteomic analysis shows that stress response proteins are significantly up-regulated in resistant diploid wheat (Triticum monococcum) in response to attack by the grain aphid (Sitobion avenae). Molecular Breeding, 35(2), https://doi.org/10.1007/s11032-015-0220-x

The grain aphid Sitobion avenae (F.) is a major pest of wheat, acting as a virus vector as well as causing direct plant damage. Commonly grown wheat varieties in the UK have only limited resistance to this pest. The present study was carried out to i... Read More about Proteomic analysis shows that stress response proteins are significantly up-regulated in resistant diploid wheat (Triticum monococcum) in response to attack by the grain aphid (Sitobion avenae).

Characterization of cellulolytic activity in the gut of the terrestrial land slug Arion ater : Biochemical identification of targets for intensive study (2014)
Journal Article
Joynson, R., Swamy, A., Bou, P., Chapuis, A., & Ferry, N. (2014). Characterization of cellulolytic activity in the gut of the terrestrial land slug Arion ater : Biochemical identification of targets for intensive study. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 177, 29-35. https://doi.org/10.1016/j.cbpb.2014.08.003

The level of cellulolytic activity in different areas of the gut of the terrestrial slug Arion ater was assayed at different temperatures and pH values. To do this, crude gut proteins were isolated and assayed using modified dinitrosalicylic acid red... Read More about Characterization of cellulolytic activity in the gut of the terrestrial land slug Arion ater : Biochemical identification of targets for intensive study.

Characterization of four midgut aminopeptidase N isozymes from Ostrinia furnacalis strains with different susceptibilities to Bacillus thuringiensis (2013)
Journal Article
Xu, L., Wang, Z., Zhang, J., Ferry, N., Edwards, M., Gatehouse, A., & He, K. (2014). Characterization of four midgut aminopeptidase N isozymes from Ostrinia furnacalis strains with different susceptibilities to Bacillus thuringiensis. Journal of Invertebrate Pathology, 115, 95-98. https://doi.org/10.1016/j.jip.2013.11.001

The full-length cDNA of four Ofapn aminopeptidases were cloned and sequenced from susceptible and resistant Ostrinia furnacalis strains. Four sequences were identified as APN because they shared the common structural features with APN from Lepidopter... Read More about Characterization of four midgut aminopeptidase N isozymes from Ostrinia furnacalis strains with different susceptibilities to Bacillus thuringiensis.

A proteomic approach to study the mechanism of tolerance to Bt toxins in Ostrinia furnacalis larvae selected for resistance to Cry1Ab (2013)
Journal Article
Xu, L., Ferry, N., Wang, Z., Zhang, J., Edwards, M. G., Gatehouse, A. M., & He, K. A proteomic approach to study the mechanism of tolerance to Bt toxins in Ostrinia furnacalis larvae selected for resistance to Cry1Ab. Transgenic Research, 22, 1155–1166. https://doi.org/10.1007/s11248-013-9718-3

A Cry1Ab-resistant population of Asian corn borer (ACB-AbR) exhibiting approximately 100 times greater resistance to activated Cry1Ab than a susceptible population (Ostrinia furnacalis; ACB-BtS), was previously shown to exhibit high levels of cross-r... Read More about A proteomic approach to study the mechanism of tolerance to Bt toxins in Ostrinia furnacalis larvae selected for resistance to Cry1Ab.