School of Science, Engineering & Environment

“Omics”-Informed drug and biomarker discovery : opportunities, challenges and future perspectives (2016)
Journal Article
Matthews, H., Hanison, J., & Nirmalan, N. (2016). “Omics”-Informed drug and biomarker discovery : opportunities, challenges and future perspectives. Proteomes, 4(3), 28. https://doi.org/10.3390/proteomes4030028

The pharmaceutical industry faces unsustainable program failure despite significant increases in investment. Dwindling discovery pipelines, rapidly expanding R&D budgets and increasing regulatory control, predict significant gaps in the future drug m... Read More about “Omics”-Informed drug and biomarker discovery : opportunities, challenges and future perspectives.

Initial development and validation of a novel extraction method for quantitative mining of the formalin-fixed, paraffin-embedded tissue proteome for biomarker investigations (2011)
Journal Article
Nirmalan, N., Hughes, C., Peng, J., McKenna, T., Langridge, J., Cairns, D., …Banks, R. (2011). Initial development and validation of a novel extraction method for quantitative mining of the formalin-fixed, paraffin-embedded tissue proteome for biomarker investigations. Journal of Proteome Research, 10(2), 896-906. https://doi.org/10.1021/pr100812d

Annotated formalin-fixed, paraffin-embedded (FFPE) tissue archives constitute a valuable resource for retrospective biomarker discovery. However, proteomic exploration of archival tissue is impeded by extensive formalin-induced covalent cross-lin... Read More about Initial development and validation of a novel extraction method for quantitative mining of the formalin-fixed, paraffin-embedded tissue proteome for biomarker investigations.

Development and validation of a novel protein extraction methodology for quantitation of protein expression in formalin-fixed paraffin-embedded tissues using western blotting (2009)
Journal Article
Nirmalan, N., Harnden, P., Selby, P., & Banks, R. (2009). Development and validation of a novel protein extraction methodology for quantitation of protein expression in formalin-fixed paraffin-embedded tissues using western blotting. The Journal of pathology, 217(4), 497-506. https://doi.org/10.1002/path.2504

The development of efficient formaldehyde cross-link reversal strategies will make the vast diagnostic tissue archives of pathology departments amenable to prospective and retrospective translational research, particularly in biomarker-driven prote... Read More about Development and validation of a novel protein extraction methodology for quantitation of protein expression in formalin-fixed paraffin-embedded tissues using western blotting.

Mining the archival formalin-fixed paraffin-embedded tissue proteome: opportunities and challenges (2008)
Journal Article
Nirmalan, N., Harnden, P., Selby, P., & Banks, R. (2008). Mining the archival formalin-fixed paraffin-embedded tissue proteome: opportunities and challenges. Molecular BioSystems, 4(7), 712-720. https://doi.org/10.1039/b800098k

The significant potential of tissue-based proteomic biomarker studies can be restricted by difficulties in accessing samples in optimal fresh-frozen form. While archival formalinfixed tissue collections with attached clinical and outcome data repre... Read More about Mining the archival formalin-fixed paraffin-embedded tissue proteome: opportunities and challenges.

Quantitative proteomics of the human malaria parasite Plasmodium falciparum and its application to studies of development and inhibition (2004)
Journal Article
Nirmalan, N., Sims, P. F. G., & Hyde, J. E. Quantitative proteomics of the human malaria parasite Plasmodium falciparum and its application to studies of development and inhibition. Molecular Microbiology, 52(4), 1187-1199. https://doi.org/10.1111/j.1365-2958.2004.04049.x

The ability to measure accurately comparative levels of protein expression after drug challenge, metabolic stress, developmental programming or other perturbation represents one of the most important goals in post-genomics malaria research. We descri... Read More about Quantitative proteomics of the human malaria parasite Plasmodium falciparum and its application to studies of development and inhibition.

Transcriptional analysis of genes encoding enzymes of the folate pathway in the human malaria parasite Plasmodium falciparum: Folate pathway transcription in malaria parasites (2002)
Journal Article
Nirmalan, N., Wang, P., Sims, P. F. G., & Hyde, J. E. (2002). Transcriptional analysis of genes encoding enzymes of the folate pathway in the human malaria parasite Plasmodium falciparum: Folate pathway transcription in malaria parasites. Molecular Microbiology, 46(1), 179-190. https://doi.org/10.1046/j.1365-2958.2002.03148.x

Folate metabolism in Plasmodium falciparum is essential for cell growth and replication, and the target of important antimalarial agents. The pathway comprises a series of enzymes that convert GTP to derivatives of tetrahydrofolate, which are cofacto... Read More about Transcriptional analysis of genes encoding enzymes of the folate pathway in the human malaria parasite Plasmodium falciparum: Folate pathway transcription in malaria parasites.

Drug repositioning as a route to anti-malarial drug discovery: preliminary investigation of the in vitro anti-malarial efficacy of emetine dihydrochloride hydrate
Journal Article
Matthews, H., Usman-Idris, M., Khan, F., Read, M., & Nirmalan, N. Drug repositioning as a route to anti-malarial drug discovery: preliminary investigation of the in vitro anti-malarial efficacy of emetine dihydrochloride hydrate. Malaria Journal, 12(1), 359. https://doi.org/10.1186/1475-2875-12-359

Background Drug repurposing or repositioning refers to the usage of existing drugs in diseases other than those it was originally used for. For diseases like malaria, where there is an urgent need for active drug candidates, the strategy offers a... Read More about Drug repositioning as a route to anti-malarial drug discovery: preliminary investigation of the in vitro anti-malarial efficacy of emetine dihydrochloride hydrate.

Outputs (18)