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NAPO as a novel marker for apoptosis

Sayan, BS; Ince, G; Sayan, AE; Ozturk, M

NAPO as a novel marker for apoptosis Thumbnail


Authors

G Ince

AE Sayan

M Ozturk



Abstract

Apoptosis or programmed cell death plays a pivotal role in embryonic development and maintenance of homeostasis. It is also involved in the etiology of pathophysiological conditions such as cancer, neurodegenerative, autoimmune, infectious, and heart diseases. Consequently, the study of apoptosis is now at center of both basic and clinical research applications. Therefore, sensitive and simple apoptosis detection techniques are required. Here we describe a monoclonal antibody–defined novel antigen, namely NAPO (negative in apoptosis), which is specifically lost during apoptosis. The anti-NAPO antibody recognizes two nuclear polypeptides of 60 and 70 kD. The antigen is maintained in quiescent and senescent cells, as well as in different phases of the cell cycle, including mitosis. Thus, immunodetection of NAPO antigen provides a specific, sensitive, and easy method for differential identification of apoptotic and nonapoptotic cells.

Citation

Sayan, B., Ince, G., Sayan, A., & Ozturk, M. (2001). NAPO as a novel marker for apoptosis. Journal of Cell Biology, 155(5), 719-724. https://doi.org/10.1083/jcb.200106044

Journal Article Type Article
Publication Date Nov 26, 2001
Deposit Date Feb 6, 2023
Publicly Available Date Feb 6, 2023
Journal Journal of Cell Biology
Print ISSN 0021-9525
Electronic ISSN 1540-8140
Publisher Rockefeller University Press
Volume 155
Issue 5
Pages 719-724
DOI https://doi.org/10.1083/jcb.200106044
Publisher URL https://doi.org/10.1083/Fjcb.200106044

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