New insights in the expression of stromal caveolin 1 in breast cancer spread to axillary lymph nodes

Scatena, C; Fanelli, G; Fanelli, GN; Menicagli, M; Aretini, P; Ortenzi, V; Civitelli, SP; Innocenti, L; Sotgia, F; Lisanti, MP; Naccarato, AG

Abstract

Recent evidence suggests that a loss of expression of caveolin in the stromal compartment (sCav-1) of human invasive breast carcinoma (IBC) may be a predictor of disease recurrence, metastasis and poor outcome. At present, there is little knowledge regarding the expression of sCav-1 at the metastatic sites. We therefore studied sCav-1 expression in IBCs and in their axillary lymph nodes to seek a correlation with cancer metastasis. 189 consecutive invasive IBCs (53 with axillary lymph node metastases and 136 without) were studied by immunohistochemistry, using a rabbit polyclonal anti-Cav-1 antibody. In IBCs sCav-1 was evaluated in fibroblasts scattered in the tumor stroma whereas in lymph nodes sCav-1 was assessed in fibroblast-like stromal cells. For the first time, we observed a statistically significant progressive loss of sCav-1 from normal/reactive axillary lymph nodes of tumors limited to the breast to metastatic axillary lymph nodes, through normal/reactive axillary lymph nodes of tumors with axillary metastatic spread. These data indicate that Cav-1 expressed by the stromal compartment of lymph nodes, somehow, may possibly contribute to metastatic spread in IBC.

Citation

Scatena, C., Fanelli, G., Fanelli, G., Menicagli, M., Aretini, P., Ortenzi, V., …Naccarato, A. (2021). New insights in the expression of stromal caveolin 1 in breast cancer spread to axillary lymph nodes. Scientific reports, 11(1), 2755. https://doi.org/10.1038/s41598-021-82405-y

• Journal Article Type: Article
• Acceptance Date: Jan 19, 2021
• Publication Date: Feb 2, 2021
• Deposit Date: Feb 3, 2021
• Publicly Available Date: Feb 3, 2021
• Journal: Scientific Reports
• Print ISSN: 2045-2322
• Publisher: Nature Publishing Group
• Volume: 11
• Issue: 1
• Pages: 2755
• DOI: https://doi.org/10.1038/s41598-021-82405-y
• Publisher URL: https://doi.org/10.1038/s41598-021-82405-y
• Related Public URLs: http://www.nature.com/srep/index.html
• Additional Information: Additional Information : ** From Springer Nature via Jisc Publications Router ** Licence for this article: http://creativecommons.org/licenses/by/4.0/ **Journal IDs: eissn 2045-2322 **Article IDs: publisher-id: s41598-021-82405-y; manuscript: 82405 **History: collection 12-2021; published 02-02-2021; online 02-02-2021; registration 20-01-2021; accepted 19-01-2021; submitted 09-05-2020