New insights in the expression of stromal caveolin 1 in breast cancer spread to axillary lymph nodes

Scatena, C; Fanelli, G; Fanelli, GN; Menicagli, M; Aretini, P; Ortenzi, V; Civitelli, SP; Innocenti, L; Sotgia, F; Lisanti, MP; Naccarato, AG

Abstract

Recent evidence suggests that a loss of expression of caveolin in the stromal compartment (sCav-1) of human invasive breast carcinoma (IBC) may be a predictor of disease recurrence, metastasis and poor outcome. At present, there is little knowledge regarding the expression of sCav-1 at the metastatic sites. We therefore studied sCav-1 expression in IBCs and in their axillary lymph nodes to seek a correlation with cancer metastasis. 189 consecutive invasive IBCs (53 with axillary lymph node metastases and 136 without) were studied by immunohistochemistry, using a rabbit polyclonal anti-Cav-1 antibody. In IBCs sCav-1 was evaluated in fibroblasts scattered in the tumor stroma whereas in lymph nodes sCav-1 was assessed in fibroblast-like stromal cells. For the first time, we observed a statistically significant progressive loss of sCav-1 from normal/reactive axillary lymph nodes of tumors limited to the breast to metastatic axillary lymph nodes, through normal/reactive axillary lymph nodes of tumors with axillary metastatic spread. These data indicate that Cav-1 expressed by the stromal compartment of lymph nodes, somehow, may possibly contribute to metastatic spread in IBC.

• Journal Article Type: Article
• Acceptance Date: Jan 19, 2021
• Publication Date: Feb 2, 2021
• Deposit Date: Feb 3, 2021
• Publicly Available Date: Feb 3, 2021
• Journal: Scientific Reports
• Print ISSN: 2045-2322
• Publisher: Nature Publishing Group
• Volume: 11
• Issue: 1
• Pages: 2755
• DOI: https://doi.org/10.1038/s41598-021-82405-y
• Publisher URL: https://doi.org/10.1038/s41598-021-82405-y
• Related Public URLs: http://www.nature.com/srep/index.html
• Additional Information: Additional Information : ** From Springer Nature via Jisc Publications Router ** Licence for this article: http://creativecommons.org/licenses/by/4.0/ **Journal IDs: eissn 2045-2322 **Article IDs: publisher-id: s41598-021-82405-y; manuscript: 82405 **History: collection 12-2021; published 02-02-2021; online 02-02-2021; registration 20-01-2021; accepted 19-01-2021; submitted 09-05-2020