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Quantum dot-based thermal spectroscopy and imaging of optically trapped microspheres and single cells

Martinez Maestro, L; Haro Gonzalez, P; Ramsay, WT; del Rosal, B; Santacruz-Gomez, K; Iglesias de la Cruz, MC; Sanz Rodríguez, F; Chooi, JY; Rodriguez Sevilla, P; Bettinelli, M; Choudhury, D; Kar, JK; Garcia Sole, J; Jaque, D; Paterson, L

Authors

L Martinez Maestro

P Haro Gonzalez

WT Ramsay

B del Rosal

K Santacruz-Gomez

MC Iglesias de la Cruz

F Sanz Rodríguez

JY Chooi

P Rodriguez Sevilla

M Bettinelli

D Choudhury

JK Kar

J Garcia Sole

D Jaque

L Paterson



Abstract

Laser‐induced thermal effects in optically trapped microspheres and single cells are investigated by quantum dot luminescence thermometry. Thermal spectroscopy has revealed a non‐localized temperature distribution around the trap that extends over tens of micrometers, in agreement with previous theoretical models besides identifying water absorption as the most important heating source. The experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This is corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. This quantum dot luminescence thermometry demonstrates that optical trapping with 820 nm laser radiation produces minimum intracellular heating, well below the cytotoxic level (43 °C), thus, avoiding cell damage.

Citation

Martinez Maestro, L., Haro Gonzalez, P., Ramsay, W., del Rosal, B., Santacruz-Gomez, K., Iglesias de la Cruz, M., …Paterson, L. (2013). Quantum dot-based thermal spectroscopy and imaging of optically trapped microspheres and single cells. Small, 9(12), 2162-2170. https://doi.org/10.1002/smll.201201740

Journal Article Type Article
Online Publication Date Feb 11, 2013
Publication Date Jun 14, 2013
Deposit Date Dec 10, 2019
Journal Small
Print ISSN 1613-6810
Electronic ISSN 1613-6829
Publisher Wiley
Volume 9
Issue 12
Pages 2162-2170
DOI https://doi.org/10.1002/smll.201201740
Publisher URL https://doi.org/10.1002/smll.201201740
Related Public URLs https://onlinelibrary.wiley.com/journal/16136829
Additional Information Funders : Universidad Autónoma de Madrid;Comunidad Autonoma de Madrid;Spanish Ministerio de Educacion y Ciencia;Caja Madrid Foundation;Fondazione Cariverona;fundacion Dr. Manuel Morales;Banco Santander and CEAL-UAM;UK Engineering and Physical Sciences Research Council (EPSRC);Heriot Watt University Life Science Interface Theme Scholarship

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