Skip to main content

Research Repository

Advanced Search

Influence of preservation methods, sample medium and sampling time on eDNA recovery in a neotropical river

Sales, NG; Wangensteen Fuentes, OS; Carvalho, DC; Mariani, S

Influence of preservation methods, sample medium and sampling time on eDNA recovery in a neotropical river Thumbnail


Authors

NG Sales

OS Wangensteen Fuentes

DC Carvalho

S Mariani



Abstract

Environmental DNA (eDNA) has rapidly emerged as a promising biodiversity monitoring technique, proving to be a sensitive and cost‐effective method for species detection. Despite the increasing popularity of eDNA, several questions regarding its limitations remain to be addressed. We investigated the effect of sampling medium and time, and preservation methods, on fish detection performance based on eDNA metabarcoding of neotropical freshwater samples. Water and sediment samples were collected from 11 sites along the Jequitinhonha River, Southeastern Brazil; sediment samples were stored in ethanol, while the same amounts of water per sample (3 L) were stored in a cool box with ice, as well as by adding the cationic surfactant benzalkonium chloride (BAC). Sediment and water samples yielded a similar amount of fish MOTUs (237 vs. 239 in the first sampling event, and 153 vs. 142 in the second sampling event). Water stored in ice provided better results than those preserved in BAC (239 and 142 vs. 194 and 71 MOTUs). While documenting the effectiveness of eDNA surveys as practical tools for fish biodiversity monitoring in poorly accessible areas, we showed that keeping water samples cooled results in greater eDNA recovery and taxon detection than by adding cationic surfactants (BAC) as sample preservatives. Furthermore, by comparing two sets of samples collected from the same locations at a 3‐week interval, we highlight the importance of conducting multiple sampling events when attempting to recover a realistic picture of fish assemblages in lotic systems.

Citation

Sales, N., Wangensteen Fuentes, O., Carvalho, D., & Mariani, S. (2019). Influence of preservation methods, sample medium and sampling time on eDNA recovery in a neotropical river. Environmental DNA, 1(2), 119-130. https://doi.org/10.1002/edn3.14

Journal Article Type Article
Acceptance Date Apr 26, 2019
Online Publication Date May 31, 2019
Publication Date May 31, 2019
Deposit Date Jun 11, 2019
Publicly Available Date Jun 11, 2019
Journal Environmental DNA
Print ISSN 2637-4943
Publisher Wiley Open Access
Volume 1
Issue 2
Pages 119-130
DOI https://doi.org/10.1002/edn3.14
Publisher URL https://doi.org/10.1002/edn3.14
Related Public URLs https://onlinelibrary.wiley.com/journal/26374943
Additional Information Funders : Conselho Nacional de Desenvolvimento Científico e Tecnológico
Projects : 204620/2014-7

Files






You might also like



Downloadable Citations