S Mariani
Sponges as natural environmental DNA samplers
Mariani, S; Baillie, CJP; Colosimo, G; Riesgo, A
Authors
CJP Baillie
G Colosimo
A Riesgo
Abstract
At a time of unprecedented impacts on marine biodiversity, scientists are rapidly becoming persuaded by the potential of screening large swathes of the oceans through the retrieval, amplification and sequencing of trace DNA fragments left behind by marine organisms; an approach known as ‘environmental DNA’ (eDNA) [1]. In trying to circumvent the many challenges associated with water filtration and DNA isolation from environmental samples, significant investment is being made in high-tech solutions, such as automated underwater vehicles and robots [2]. Here, instead, we explored a simpler, alternative option, based on the recovery of eDNA from sponges (phylum Porifera), the planet’s most effective water-filterers. We obtained sponge samples from Mediterranean and Antarctic surveys, extracted total DNA from their tissues, and obtained tens of thousands of fish DNA reads via metabarcoding, which were able to clearly distinguish samples from the two regions. One Antarctic sample yielded hundreds of reads from chinstrap penguin (Pygoscelis antarcticus) and Weddell seal (Leptonychotes weddellii). We argue that this ‘natural sampler DNA’ (nsDNA) approach is poised to become a powerful, affordable, universal tool for aquatic biodiversity monitoring globally.
Citation
Mariani, S., Baillie, C., Colosimo, G., & Riesgo, A. (2019). Sponges as natural environmental DNA samplers. Current Biology, 29(11), R401-R402. https://doi.org/10.1016/j.cub.2019.04.031
Journal Article Type | Article |
---|---|
Acceptance Date | Apr 19, 2019 |
Online Publication Date | Jun 3, 2019 |
Publication Date | Jun 3, 2019 |
Deposit Date | Jun 5, 2019 |
Publicly Available Date | Jun 5, 2019 |
Journal | Current Biology |
Print ISSN | 0960-9822 |
Publisher | Elsevier |
Volume | 29 |
Issue | 11 |
Pages | R401-R402 |
DOI | https://doi.org/10.1016/j.cub.2019.04.031 |
Publisher URL | https://doi.org/10.1016/j.cub.2019.04.031 |
Related Public URLs | https://www.cell.com/current-biology/home |
Files
PIIS0960982219304294.pdf
(402 Kb)
PDF
Licence
http://creativecommons.org/licenses/by/4.0/
Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
You might also like
Estuarine molecular bycatch as a landscape-wide biomonitoring tool
(2021)
Journal Article
Space-time dynamics in monitoring neotropical fish communities using eDNA metabarcoding
(2020)
Journal Article
Downloadable Citations
About USIR
Administrator e-mail: library-research@salford.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search