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Skin swabs with FTA® cards as a dry storage source for amphibian DNA

Ward, A; Hide, G; Jehle, R

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Authors

A Ward



Abstract

Amphibians are the most endangered group of vertebrates, and conservation measures increasingly rely on information drawn from genetic markers. The present study explores skin swabs with Whatman FTA® cards as a method to retrieve PCR-amplifiable amphibian DNA. Swabs from ten adult great crested newts (Triturus cristatus) were used to compare FTA® card-based protocols with tissue sampling based on toe clips. PCR success rates were measured for seven microsatellite markers and one mtDNA marker (ND4) after 6 months of sample storage. We demonstrate that the merging of eight FTA® card punches from Qiagen-based DNA extraction always led to successful amplifications in at least one replicate, at an overall PCR success rate of 78%. The newly established protocol has the potential for wide application to future DNA-based amphibian studies.

Citation

Ward, A., Hide, G., & Jehle, R. (2019). Skin swabs with FTA® cards as a dry storage source for amphibian DNA. Conservation Genetics Resources, 11, 309-311. https://doi.org/10.1007/s12686-018-1018-z

Journal Article Type Article
Acceptance Date Mar 1, 2018
Online Publication Date Mar 2, 2018
Publication Date Sep 15, 2019
Deposit Date Mar 5, 2018
Publicly Available Date Mar 5, 2018
Journal Conservation Genetics Resources
Print ISSN 1877-7252
Electronic ISSN 1877-7260
Publisher Springer Verlag
Volume 11
Pages 309-311
DOI https://doi.org/10.1007/s12686-018-1018-z
Publisher URL http://dx.doi.org/10.1007/s12686-018-1018-z
Related Public URLs https://link.springer.com/journal/12686

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