Bio-inspired artemether loaded human serum albumin nanoparticles for effective control of malaria-infected erythrocytes

Sidhaye, AA; Bhuran, KC; Zambare, S; Abubaker, M; Nirmalan, NJ; Singh, KK

Abstract

Background: The intra-erythrocytic development of the malarial parasite is dependent on active uptake of nutrients, including human serum albumin(HSA), into parasitized erythrocytes(pRBCs). We have designed HSA-based nanoparticles as a potential drugdelivery
option for antimalarials.
Methods: Artemether-loaded nanoparticles(AAN) were designed and antimalarial activity evaluated in-vitro/in-vivo using Plasmodium falciparum/Plasmodium berghei species, respectively.
Results: Selective internalisation of AAN into Plasmodium-infected RBCs in preference to healthy erythrocytes was observed using confocal-imaging. In-vitro studies showed 50% dose reduction for AAN as compared to drug-only controls to achieve IC50 levels of inhibition. The nanoparticles exhibited 2-fold higher peak drug concentrations in RBCs with antimalarial activity at 50% of therapeutic doses in P.bergei-infected mice.
Conclusion: HSA-based nanoparticles offer safe and
effective approach for selective targeting of antimalarial drugs.

Citation

Sidhaye, A., Bhuran, K., Zambare, S., Abubaker, M., Nirmalan, N., & Singh, K. (2016). Bio-inspired artemether loaded human serum albumin nanoparticles for effective control of malaria-infected erythrocytes. Nanomedicine, 11(21), 2809-2828. https://doi.org/10.2217/nnm-2016-0235

• Journal Article Type: Article
• Acceptance Date: Aug 30, 2016
• Online Publication Date: Oct 19, 2016
• Publication Date: Nov 1, 2016
• Deposit Date: Jun 5, 2017
• Publicly Available Date: Nov 30, 2017
• Journal: Nanomedicine
• Print ISSN: 1743-5889
• Publisher: Future Medicine
• Volume: 11
• Issue: 21
• Pages: 2809-2828
• DOI: https://doi.org/10.2217/nnm-2016-0235
• Publisher URL: http://dx.doi.org/10.2217/nnm-2016-0235
• Related Public URLs: http://www.futuremedicine.com/loi/nnm?close=2017