Direct surface-enhanced raman scattering analysis of DNA duplexes

Guerrini, L; Krpetic, Z; Van Lierop, D; Alvarez-Puebla, RA; Graham, D

doi:10.1002/anie.201408558

Direct surface-enhanced raman scattering analysis of DNA duplexes

Guerrini, L; Krpetic, Z; Van Lierop, D; Alvarez-Puebla, RA; Graham, D

Authors

L Guerrini

Dr Zeljka Krpetic Z.Krpetic@salford.ac.uk
Associate Professor/Reader

D Van Lierop

RA Alvarez-Puebla

D Graham

Abstract

The exploration of the genetic information carried by DNA has become a major scientific challenge. Routine DNA analysis, such as PCR, still suffers from important intrinsic limitations. Surface-enhanced Raman spectroscopy (SERS) has emerged as an outstanding opportunity for the development of DNA analysis, but its application to duplexes (dsDNA) has been largely hampered by reproducibility and/or sensitivity issues. A simple strategy is presented to perform ultrasensitive direct label-free analysis of unmodified dsDNA with the means of SERS by using positively charged silver colloids. Electrostatic adhesion of DNA promotes nanoparticle aggregation into stable clusters yielding intense and reproducible SERS spectra at nanogram level. As potential applications, we report the quantitative recognition of hybridization events as well as the first examples of SERS recognition of single base mismatches and base methylations (5-methylated cytosine and N6-methylated Adenine) in duplexes.

Citation

Guerrini, L., Krpetic, Z., Van Lierop, D., Alvarez-Puebla, R., & Graham, D. (2014). Direct surface-enhanced raman scattering analysis of DNA duplexes. Angewandte Chemie International Edition, 54(4), 1144-1148. https://doi.org/10.1002/anie.201408558

Journal Article Type	Article
Publication Date	Nov 20, 2014
Deposit Date	Oct 6, 2016
Journal	Angewandte Chemie International Edition
Print ISSN	1433-7851
Publisher	Wiley
Volume	54
Issue	4
Pages	1144-1148
DOI	https://doi.org/10.1002/anie.201408558
Publisher URL	http://dx.doi.org/10.1002/anie.201408558
Related Public URLs	http://onlinelibrary.wiley.com/doi/10.1002/anie.201408558/abstract
Additional Information	Funders : Spanish Ministerio de Economia y Competitividad;European Research Council;PrioSERS Projects : CrossSERS;PrioSERS Grant Number: CTQ2011-23167 Grant Number: FP7/2013 329131 Grant Number: FP7/2014 623527