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Direct surface-enhanced raman scattering analysis of DNA duplexes

Guerrini, L; Krpetic, Z; Van Lierop, D; Alvarez-Puebla, RA; Graham, D

Authors

L Guerrini

D Van Lierop

RA Alvarez-Puebla

D Graham



Abstract

The exploration of the genetic information carried by DNA has become a major scientific challenge. Routine DNA analysis, such as PCR, still suffers from important intrinsic limitations. Surface-enhanced Raman spectroscopy (SERS) has emerged as an outstanding opportunity for the development of DNA analysis, but its application to duplexes (dsDNA) has been largely hampered by reproducibility and/or sensitivity issues. A simple strategy is presented to perform ultrasensitive direct label-free analysis of unmodified dsDNA with the means of SERS by using positively charged silver colloids. Electrostatic adhesion of DNA promotes nanoparticle aggregation into stable clusters yielding intense and reproducible SERS spectra at nanogram level. As potential applications, we report the quantitative recognition of hybridization events as well as the first examples of SERS recognition of single base mismatches and base methylations (5-methylated cytosine and N6-methylated Adenine) in duplexes.

Citation

Guerrini, L., Krpetic, Z., Van Lierop, D., Alvarez-Puebla, R., & Graham, D. (2014). Direct surface-enhanced raman scattering analysis of DNA duplexes. Angewandte Chemie International Edition, 54(4), 1144-1148. https://doi.org/10.1002/anie.201408558

Journal Article Type Article
Publication Date Nov 20, 2014
Deposit Date Oct 6, 2016
Journal Angewandte Chemie International Edition
Print ISSN 1433-7851
Publisher Wiley
Volume 54
Issue 4
Pages 1144-1148
DOI https://doi.org/10.1002/anie.201408558
Publisher URL http://dx.doi.org/10.1002/anie.201408558
Related Public URLs http://onlinelibrary.wiley.com/doi/10.1002/anie.201408558/abstract
Additional Information Funders : Spanish Ministerio de Economia y Competitividad;European Research Council;PrioSERS
Projects : CrossSERS;PrioSERS
Grant Number: CTQ2011-23167
Grant Number: FP7/2013 329131
Grant Number: FP7/2014 623527