L Guerrini
Direct surface-enhanced raman scattering analysis of DNA duplexes
Guerrini, L; Krpetic, Z; Van Lierop, D; Alvarez-Puebla, RA; Graham, D
Authors
Dr Zeljka Krpetic Z.Krpetic@salford.ac.uk
Associate Professor/Reader
D Van Lierop
RA Alvarez-Puebla
D Graham
Abstract
The exploration of the genetic information carried by DNA has become a major scientific challenge. Routine DNA analysis, such as PCR, still suffers from important intrinsic limitations. Surface-enhanced Raman spectroscopy (SERS) has emerged as an outstanding opportunity for the development of DNA analysis, but its application to duplexes (dsDNA) has been largely hampered by reproducibility and/or sensitivity issues. A simple strategy is presented to perform ultrasensitive direct label-free analysis of unmodified dsDNA with the means of SERS by using positively charged silver colloids. Electrostatic adhesion of DNA promotes nanoparticle aggregation into stable clusters yielding intense and reproducible SERS spectra at nanogram level. As potential applications, we report the quantitative recognition of hybridization events as well as the first examples of SERS recognition of single base mismatches and base methylations (5-methylated cytosine and N6-methylated Adenine) in duplexes.
Citation
Guerrini, L., Krpetic, Z., Van Lierop, D., Alvarez-Puebla, R., & Graham, D. (2014). Direct surface-enhanced raman scattering analysis of DNA duplexes. Angewandte Chemie International Edition, 54(4), 1144-1148. https://doi.org/10.1002/anie.201408558
Journal Article Type | Article |
---|---|
Publication Date | Nov 20, 2014 |
Deposit Date | Oct 6, 2016 |
Journal | Angewandte Chemie International Edition |
Print ISSN | 1433-7851 |
Publisher | Wiley |
Volume | 54 |
Issue | 4 |
Pages | 1144-1148 |
DOI | https://doi.org/10.1002/anie.201408558 |
Publisher URL | http://dx.doi.org/10.1002/anie.201408558 |
Related Public URLs | http://onlinelibrary.wiley.com/doi/10.1002/anie.201408558/abstract |
Additional Information | Funders : Spanish Ministerio de Economia y Competitividad;European Research Council;PrioSERS Projects : CrossSERS;PrioSERS Grant Number: CTQ2011-23167 Grant Number: FP7/2013 329131 Grant Number: FP7/2014 623527 |
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