Antibacterial efficacy of a cetylpyridinium chloride-based mouthrinse against Fusobacterium nucleatum and in vitro plaques

Latimer, J; Munday, JL; Buzza, KM; Sreenivasan, PK; McBain, AJ

Abstract

PURPOSE

To assess the antimicrobial effects of a fluoride-free and alcohol-free mouthrinse containing 0.075% CPC (test rinse, TR) compared with an otherwise-identical CPC-free control rinse (CR).

METHODS

Activity against laboratory cultures of Fusobacterium nucleatum, a bacterium associated with gingival disease, was determined using viable counting following 30-second exposures to TR and CR. Effects against intact saliva-derived plaque biofilms were quantified using confocal microscopy coupled with three-dimensional image analyses (viability profiling).

RESULTS

Short exposures to TR caused significant inactivation of F. nucleatum, as determined by viable counting (c. 3 log reduction compared to the control rinse, P < 0.05). Confocal microscopy revealed extensive inactivation of complex oral biofilms following treatment with TR; biofilms were significantly less viable than those exposed to CR and three-dimensional images revealed extensive zones of dead bacteria even within plaque depths. In conclusion, this investigation demonstrates that the CPC-containing mouthrinse has significant antibacterial efficacy against oral bacteria associated with gingival disease and significantly inactivated plaque biofilm in comparison to a relevant control.

Citation

Latimer, J., Munday, J., Buzza, K., Sreenivasan, P., & McBain, A. (2015). Antibacterial efficacy of a cetylpyridinium chloride-based mouthrinse against Fusobacterium nucleatum and in vitro plaques. American journal of dentistry, 28 Spe, 9A-13A

• Journal Article Type: Article
• Publication Date: Sep 1, 2015
• Deposit Date: Oct 24, 2016
• Journal: American Journal of Dentistry
• Print ISSN: 0894-8275
• Volume: 28 Spe
• Pages: 9A-13A
• Additional Information: Funders : Colgate Palmolive