Further evidence from SSCP and ITS DNA sequencing support Trypanosoma evansi and Trypanosoma equiperdum as subspecies or even strains of Trypanosoma brucei

Wen, Y; Lun, Z; Zhu, X; Hide, G; Lai, D

Abstract

The subgenus Trypanozoon includes three species Trypanosoma brucei, Trypanosoma evansi and Trypanosoma equiperdum, which are morphologically identical and indistinguishable even using some molecular methods. In this study, PCR-based single strand conformation polymorphism (PCR-SSCP) was used to analyze the ribosomal DNA of the Trypanozoon species. Data indicate different patterns of ITS2 fragments between T. brucei, T. evansi and T. equiperdum by SSCP. Furthermore, analysis of total ITS sequences within these three members of the subgenus Trypanozoon showed a high degree of homology using phylogenetic analysis but were polyphyletic in haplotype networks. These data provide novel nuclear evidence to further support the notion that T. evansi and T. equiperdum should be subspecies or even strains of T. brucei.

• Journal Article Type: Article
• Acceptance Date: Mar 21, 2016
• Online Publication Date: Mar 23, 2016
• Publication Date: Jul 1, 2016
• Deposit Date: Apr 6, 2016
• Publicly Available Date: Mar 23, 2017
• Journal: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
• Print ISSN: 1567-1348
• Electronic ISSN: 1567-7257
• Publisher: Elsevier
• Volume: 41
• Pages: 56-62
• DOI: https://doi.org/10.1016/j.meegid.2016.03.022
• Publisher URL: http://dx.doi.org/10.1016/j.meegid.2016.03.022
• Related Public URLs: http://www.sciencedirect.com/science/article/pii/S1567134816300910; http://ac.els-cdn.com/S1567134816300910/1-s2.0-S1567134816300910-main.pdf?_tid=ecfe90c2-fbc9-11e5-8ead-00000aacb35e&acdnat=1459928218_bad77b864fa65622240e77fdfa7afb47
• Additional Information: Funders : National Sciences Foundation of China;Guangzhou Science Technology and Innovation Commission;Natural Science Foundation of Guangdong Province;Fundamental Research Funds for the Central University; Projects : Further evidence from SSCP and ITS DNA sequencing support Trypanosoma evansi and Trypanosoma equiperdum as subspecies or even strains of Trypanosoma brucei; Grant Number: 31272305; Grant Number: 31472058; Grant Number: 31301876; Grant Number: 31402029; Grant Number: 201506010011; Grant Number: 2014A030313164; Grant Number: 2015A030311042; Grant Number: 141gpy23